Combination of adenoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes

This study aims to investigate the effect of adenoviral vector-mediated neurotrophine-3 (NT-3) gene transfer and retinoic acid (RA) pretreatment on inducing neuronal differentiation of bone marrow mesenchymal stem cells (MSCs) in vitro. MSCs could be efficiently transduced by NT-3 gene via recombina...

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Bibliographic Details
Published in:Neuroscience letters 2006-11, Vol.408 (2), p.98-103
Main Authors: Zhang, Wei, Zeng, Yuan-Shan, Zhang, Xue-Bao, Wang, Jun-Mei, Chen, Shui-Jun
Format: Article
Language:English
Subjects:
Adenoviral vector
Adenoviridae - genetics
Adenoviridae - metabolism
Animals
Antineoplastic Agents - pharmacology
Biological and medical sciences
Biomarkers - metabolism
Bone marrow cells
Bone Marrow Cells - cytology
Bone Marrow Cells - drug effects
Bone Marrow Cells - physiology
Cell Differentiation
Cell Lineage
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
Genetic Vectors
Humans
Male
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - physiology
Neuronal differentiation
Neurons - cytology
Neurons - physiology
Neurotrophin 3 - genetics
Neurotrophine-3
Phenotype
Rats
Rats, Sprague-Dawley
Receptor, trkC - genetics
Receptor, trkC - metabolism
Retinoic acid
Tretinoin - pharmacology
Vertebrates: nervous system and sense organs
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Summary:This study aims to investigate the effect of adenoviral vector-mediated neurotrophine-3 (NT-3) gene transfer and retinoic acid (RA) pretreatment on inducing neuronal differentiation of bone marrow mesenchymal stem cells (MSCs) in vitro. MSCs could be efficiently transduced by NT-3 gene via recombinant adenoviral vectors (Adv). Combination of AdvNT-3 and RA significantly promoted MSCs to differentiate into cell types associated with phenotypes of neural lineages, which included neural markers nestin, NF, MAP2 and PSD95 as detected by immunocytochemistry. But the expressions of GFAP in these cells were not obvious. RT-PCR analysis revealed that AdvNT-3 in combination with RA pretreatment could initiate the transcription of TrkC mRNA. These results demonstrate that the combination of AdvNT-3 and RA pretreatment may promote neuronal differentiation of MSCs, which may serve as ideal seed cells for the repair of spinal cord injury.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2006.08.079