A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7

In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allo...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2007-02, Vol.22 (7), p.1550-1555
Main Authors: Hou, Yanxia, Jaffrezic-Renault, Nicole, Martelet, Claude, Zhang, Aidong, Minic-Vidic, Jasmina, Gorojankina, Tatiana, Persuy, Marie-Annick, Pajot-Augy, Edith, Salesse, Roland, Akimov, Vladimir, Reggiani, Lino, Pennetta, Cecilia, Alfinito, Eleonora, Ruiz, Oscar, Gomila, Gabriel, Samitier, Josep, Errachid, Abdelhamid
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques
Biosensor
Electric Impedance
Electrochemical impedance spectroscopy
G protein-coupled receptors
Odorants - analysis
Olfactory receptor I7
Protein Engineering
Rats
Receptors, Odorant
Self-assembled multilayer
Spectrum Analysis
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Summary:In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2006.06.018