Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei

Response surface methodology (RSM) was applied to optimize the critical medium ingredients of Agaricus blazei. A three-level Box-Behnken factorial design was employed to determine the maximum biomass and extracellular polysaccharide (EPS) yields at optimum levels for glucose, yeast extract (YE), and...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2007-02, Vol.74 (1), p.78-83
Main Authors: LIU, Gao-Qiang, WANG, Xiao-Ling
Format: Article
Language:English
Subjects:
Agaricus - growth & development
Agaricus - metabolism
Agaricus blazei
Bacteria
Biological and medical sciences
Biomass
Bioreactors
Biotechnology
Biotechnology - methods
Culture Media - chemistry
Fermentation
Fundamental and applied biological sciences. Psychology
Glucose
Mathematical models
Microbiology
Models, Biological
Optimization
Peptones
Polysaccharides - biosynthesis
Yeasts
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Summary:Response surface methodology (RSM) was applied to optimize the critical medium ingredients of Agaricus blazei. A three-level Box-Behnken factorial design was employed to determine the maximum biomass and extracellular polysaccharide (EPS) yields at optimum levels for glucose, yeast extract (YE), and peptone. A mathematical model was then developed to show the effect of each medium composition and its interactions on the production of mycelial biomass and EPS. The model predicted the maximum biomass yield of 10.86 g/l that appeared at glucose, YE, peptone of 26.3, 6.84, and 6.62 g/l, respectively, while a maximum EPS yield of 348.4 mg/l appeared at glucose, YE, peptone of 28.4, 4.96, 5.60 g/l, respectively. These predicted values were also verified by validation experiments. The excellent correlation between predicted and measured values of each model justifies the validity of both the response models. The results of bioreactor fermentation also show that the optimized culture medium enhanced both biomass (13.91 +/- 0.71 g/l) and EPS (363 +/- 4.1 mg/l) production by Agaricus blazei in a large-scale fermentation process.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-006-0661-6