Sol–gel derived aluminosilicate coatings on alumina as substrate for osteoblasts

• Published in: Acta biomaterialia 2006-11, Vol.2 (6), p.659-668
• Main Authors: Leivo, Jarkko, Meretoja, Ville, Vippola, Minnamari, Levänen, Erkki, Vallittu, Pekka, Mäntylä, Tapio A.
• Format: Article
• Language: English
• Subjects: Aluminosilicate; Aluminum Oxide - chemistry; Aluminum Silicates - chemistry; Animals; Cell culture; Cell Culture Techniques - methods; Cell Differentiation; Cell Proliferation; Cell Survival; Cells, Cultured; Ceramic structure; Coated Materials, Biocompatible - chemistry; Gels - chemistry; Male; Materials Testing; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Osteoblast; Osteoblasts - cytology; Osteoblasts - physiology; Phase Transition; Rats; Rats, Sprague-Dawley; Sol–gel techniques; Tissue Engineering - methods
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2006.06.001

Rat bone marrow stromal cell differentiation on aluminosilicate 3Al 2O 3–2SiO 2 coatings was investigated. Thin ceramic coatings were prepared on α-alumina substrates by the sol–gel process and calcined in order to establish an amorphous aluminosilicate ceramic phase with and without nanosized transitional mullite crystals. In addition, coatings of thermally sprayed aluminosilicate and diphasic γ-alumina–silica nanosized colloids were prepared. Cell culture testing by rat osteoblasts showed good biocompatibility for aluminosilicates with sustained normal osteoblast functions. Despite mutual disparities in physical and chemical nanostructures, the culture findings suggested fairly similar osteoblast response to all tested coatings. The results suggest that topographical frequency parameters and chemical uniformity are important parameters in determining the best conditions for osteoblasts on sol–gel derived aluminosilicate materials.