Marker-assisted selection to improve drought adaptation in maize: the backcross approach, perspectives, limitations, and alternatives

A number of different marker-assisted selection (MAS) approaches do exist for the improvement of polygenic traits. Results of a marker-assisted backcross (MABC) selection experiment aimed at improving grain yield under drought conditions in tropical maize are presented and compared with alternative...

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Published in:Journal of experimental botany 2007-01, Vol.58 (2), p.351-360
Main Authors: Ribaut, Jean-Marcel, Ragot, Michel
Format: Article
Language:English
Subjects:
Adaptation
Agronomy. Soil science and plant productions
Alleles
Backcross
Biological and medical sciences
Corn
Crosses, Genetic
Disasters
Drought
Fundamental and applied biological sciences. Psychology
Genetic engineering applications
Genetic loci
Genetic Markers
Genetics and breeding of economic plants
Genotypes
Hybridity
marker-assisted selection
Molecular genetics
Phenotypic traits
Plant breeding: fundamental aspects and methodology
Plants
Quantitative trait loci
recurrent selection
Selection, Genetic
SPECIAL ISSUE PAPER
Water - metabolism
Zea mays
Zea mays - genetics
Zea mays - metabolism
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Ragot, Michel
description A number of different marker-assisted selection (MAS) approaches do exist for the improvement of polygenic traits. Results of a marker-assisted backcross (MABC) selection experiment aimed at improving grain yield under drought conditions in tropical maize are presented and compared with alternative MAS strategies. The introgression of favourable alleles at five target regions involved in the expression of yield components and flowering traits increased grain yield and reduced the asynchrony between male and female flowering under water-limited conditions. Eighty-five per cent of the recurrent parent's genotype at non-target loci was recovered in only four generations of MABC by screening large segregating populations (2200 individuals) for three of the four generations. Selected MABC-derived BC2F3 families were crossed with two testers and evaluated under different water regimes. Mean grain yield of MABC-derived hybrids was consistently higher than that of control hybrids (crosses from the recurrent parent to the same two testers as the MABC-derived families) under severe water stress conditions. Under those conditions, the best five MABC-derived hybrids yielded, on average, at least 50% more than control hybrids. Under mild water stress, defined as resulting in
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Results of a marker-assisted backcross (MABC) selection experiment aimed at improving grain yield under drought conditions in tropical maize are presented and compared with alternative MAS strategies. The introgression of favourable alleles at five target regions involved in the expression of yield components and flowering traits increased grain yield and reduced the asynchrony between male and female flowering under water-limited conditions. Eighty-five per cent of the recurrent parent's genotype at non-target loci was recovered in only four generations of MABC by screening large segregating populations (2200 individuals) for three of the four generations. Selected MABC-derived BC2F3 families were crossed with two testers and evaluated under different water regimes. Mean grain yield of MABC-derived hybrids was consistently higher than that of control hybrids (crosses from the recurrent parent to the same two testers as the MABC-derived families) under severe water stress conditions. 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Results of a marker-assisted backcross (MABC) selection experiment aimed at improving grain yield under drought conditions in tropical maize are presented and compared with alternative MAS strategies. The introgression of favourable alleles at five target regions involved in the expression of yield components and flowering traits increased grain yield and reduced the asynchrony between male and female flowering under water-limited conditions. Eighty-five per cent of the recurrent parent's genotype at non-target loci was recovered in only four generations of MABC by screening large segregating populations (2200 individuals) for three of the four generations. Selected MABC-derived BC2F3 families were crossed with two testers and evaluated under different water regimes. Mean grain yield of MABC-derived hybrids was consistently higher than that of control hybrids (crosses from the recurrent parent to the same two testers as the MABC-derived families) under severe water stress conditions. 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Under those conditions, the best five MABC-derived hybrids yielded, on average, at least 50% more than control hybrids. Under mild water stress, defined as resulting in &lt;50% yield reduction, no difference was observed between MABC-derived hybrids and the control plants, thus confirming that the genetic regulation for drought tolerance is dependent on stress intensity. MABC conversions involving several target regions are likely to result in partial rather than complete line conversion. Simulations were conducted to assess the utility of such partial conversions, i.e. containing favourable donor alleles at non-target regions, for subsequent phenotypic selection. The results clearly showed that selecting several genotypes (10–20) at each MABC cycle was most efficient. In the light of these results, alternative approaches to MABC are discussed, including recurrent selection, illustrated by an example of improving the adaptation of maize to low temperatures. 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source Oxford Journals Online; JSTOR Archival Journals
subjects Adaptation
Agronomy. Soil science and plant productions
Alleles
Backcross
Biological and medical sciences
Corn
Crosses, Genetic
Disasters
Drought
Fundamental and applied biological sciences. Psychology
Genetic engineering applications
Genetic loci
Genetic Markers
Genetics and breeding of economic plants
Genotypes
Hybridity
marker-assisted selection
Molecular genetics
Phenotypic traits
Plant breeding: fundamental aspects and methodology
Plants
Quantitative trait loci
recurrent selection
Selection, Genetic
SPECIAL ISSUE PAPER
Water - metabolism
Zea mays
Zea mays - genetics
Zea mays - metabolism
title Marker-assisted selection to improve drought adaptation in maize: the backcross approach, perspectives, limitations, and alternatives
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