Influence of in vitro oxygen concentrations on preimplantation embryo development, gene expression and production of hanwoo calves following embryo transfer

This study evaluated the effects of two different oxygen (O2) concentrations on in vitro embryo development, embryo quality, and gene expression and the in vivo development following embryos transfer to recipients of natural and synchronized estrus in bovines. Cumulus oocyte complexes were in vitro...

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Bibliographic Details
Published in:Molecular reproduction and development 2007-04, Vol.74 (4), p.486-496
Main Authors: Rho, Gyu-Jin, S, Balasubramanian, Kim, Dong-Sik, Son, Woo-Jin, Cho, Sang-Rae, Kim, Jung-Gon, B, Mohana kumar, Choe, Sang-Young
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Blastocyst - drug effects
Body Weight
Cattle
Embryo Transfer
Embryonic Development - drug effects
Female
Fertilization in Vitro
Freemartinism
gene expression
Gene Expression - drug effects
Gynecology. Andrology. Obstetrics
IVP embryos
Male
Medical sciences
Oxygen - pharmacology
Pregnancy
Pregnancy, Animal
Sterility. Assisted procreation
transfer
Twins
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Summary:This study evaluated the effects of two different oxygen (O2) concentrations on in vitro embryo development, embryo quality, and gene expression and the in vivo development following embryos transfer to recipients of natural and synchronized estrus in bovines. Cumulus oocyte complexes were in vitro matured in TCM199 supplemented with FSH (10 µg/ml), LH (10 µg/ml), and 10% (v/v) FBS. Presumptive zygotes were cultured in SOF medium either under 5% (low) or 20% (high) O2 in air. Cleavage rates did not differ between groups. Blastocyst and hatched blastocyst development in 5% O2 were significantly (P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20502