Elementary Steps of Enzymatic Oxidation of Nifedipine Catalyzed by Horseradish Peroxidase

The elementary steps of the enzymatic oxidation of nifedipine (NF) catalyzed by horseradish peroxidase (HRP) have been described based on analysis of kinetic magnetic field effects (MFEs). It has been shown that the first step of the catalytic cycle is single electron transfer resulting in formation...

Full description

Saved in:
Bibliographic Details
Published in:The journal of physical chemistry. B 2006-10, Vol.110 (42), p.21232-21237
Main Authors: Afanasyeva, Maria S, Taraban, Marc B, Polyakov, Nikolay E, Purtov, Peter A, Leshina, Tatyana V, Grissom, Charles B
Format: Article
Language:English
Subjects:
Catalysis
Electrons
Horseradish Peroxidase - metabolism
Kinetics
NAD
Nifedipine - metabolism
Oxidation-Reduction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The elementary steps of the enzymatic oxidation of nifedipine (NF) catalyzed by horseradish peroxidase (HRP) have been described based on analysis of kinetic magnetic field effects (MFEs). It has been shown that the first step of the catalytic cycle is single electron transfer resulting in formation of NF•+ radical cation and ferroperoxidase (Per2+). As a result, comparison with an earlier studied oxidation reaction of NADH catalyzed by HRP evidenced that the enzymatic oxidations of two substratesnative, NADH, and its synthetic analogue, NFcatalyzed by HRP in the absence of H2O2 follow identical mechanisms.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp064785s