Construction of mathematical model for high-level expression of foreign genes in pPIC9 vector and its verification

In this report, we introduced a mathematical model for high-level expression of foreign genes in pPIC9 vector. At first, we collected 40 heterologous genes expressed in pPIC9 vector, and these 40 genes were classified into high-level expression group (expression level >100mg/L, 12 genes) and low-...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-03, Vol.354 (2), p.498-504
Main Authors: Wu, Bingli, Cha, Lei, Du, Zepeng, Ying, Xiaomin, Li, Hua, Xu, Liyan, Zheng, Xiaofei, Li, Enmin, Li, Wuju
Format: Article
Language:English
Subjects:
Cloning, Molecular
Gene Expression Profiling
Gene Expression Regulation, Fungal - physiology
Genetic Vectors
Models, Genetic
Nucleic Acid Conformation
Pichia - genetics
Pichia pastoris
Plasmids
Predictive Value of Tests
Protein expression
RNA - biosynthesis
RNA - genetics
RNA secondary structure
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Summary:In this report, we introduced a mathematical model for high-level expression of foreign genes in pPIC9 vector. At first, we collected 40 heterologous genes expressed in pPIC9 vector, and these 40 genes were classified into high-level expression group (expression level >100mg/L, 12 genes) and low-level expression group (expression level
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.01.002