A novel approach for identification of tumor‐associated antigens expressed on the surface of tumor cells

To improve tumor targeting in a subset of patients, where tumor cells do not express the well‐known tumor antigens widely used in immunotherapy, we have developed a novel biotechnological tool. It is useful for tumors of various origins for the identification of tumor‐associated proteins, which are...

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Bibliographic Details
Published in:International journal of cancer 2007-03, Vol.120 (6), p.1293-1303
Main Authors: Di Cristina, Manlio, Minenkova, Olga, Pavoni, Emiliano, Beghetto, Elisa, Spadoni, Andrea, Felici, Franco, Gargano, Nicola
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - analysis
Antigens, Neoplasm - genetics
Antigens, Neoplasm - isolation & purification
Biological and medical sciences
Biotechnology - methods
breast cancer
Breast Neoplasms - chemistry
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Cell Line, Tumor
Cell Membrane - chemistry
Gene Expression
Gene Library
Gynecology. Andrology. Obstetrics
Humans
Mammary gland diseases
Medical sciences
Neoplasm Proteins - analysis
Neoplasm Proteins - genetics
Neoplasm Proteins - isolation & purification
Neoplasms - chemistry
Neoplasms - genetics
Neoplasms - metabolism
Nucleic Acid Hybridization - methods
surface antigen
transmembrane trapping
tumor targeting
Tumors
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Summary:To improve tumor targeting in a subset of patients, where tumor cells do not express the well‐known tumor antigens widely used in immunotherapy, we have developed a novel biotechnological tool. It is useful for tumors of various origins for the identification of tumor‐associated proteins, which are differentially expressed in tumor cells with respect to normal tissue, and exposed on the cell surface. For this purpose, a combination of techniques, such as “suppression subtractive hybridization” and “transmembrane trapping,” was employed. In applying this novel approach to breast cancer, we identified a large panel of cDNA fragments encoding for the well‐known tumor‐associated surface antigens, such as erb‐B2, erbB3 and the urokinase receptor and, more importantly, for several clones overexpressed in breast cancer, whose cDNA fragments match the sequences of hypothetical transmembrane proteins with unknown function. The latter may represent novel tumor‐specific targets. © 2006 Wiley‐Liss, Inc.
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.22395