Phosphoinositide synthesis and degradation in isolated rat liver peroxisomes

Analyzing peroxisomal phosphoinositide (PId #) synthesis in highly purified rat liver peroxisomes we found synthesis of phosphatidylinositol 4-phosphate (PtdIns4 P), PtdIns(4,5) P 2 and PtdIns(3,5) P 2. PtdIns3 P was hardly detected in vitro, however, was observed in vivo after [ 32P]-phosphate labe...

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Bibliographic Details
Published in:FEBS letters 2006-10, Vol.580 (25), p.5917-5924
Main Authors: Jeynov, Boyan, Lay, Dorothee, Schmidt, Frank, Tahirovic, Sabina, Just, Wilhelm W.
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 4-Kinase - metabolism
ADP ribosylation factor
Animals
ARF
Cells, Cultured
Endoplasmic Reticulum - metabolism
Hepatocytes - metabolism
In Vitro Techniques
peroxisomal membrane protein
Peroxisomes
Peroxisomes - metabolism
phosphatidic acid
phosphatidylinositol
Phosphatidylinositol kinases
Phosphatidylinositol phosphatases
Phosphatidylinositol Phosphates - biosynthesis
Phosphatidylinositol Phosphates - metabolism
phosphatidylinositol(3,5)-bisphosphate
phosphatidylinositol(4,5)-bisphosphate
phosphatidylinositol-phosphate
Phosphatidylinositols - biosynthesis
Phosphatidylinositols - metabolism
phosphoinositide
Phosphoinositide bisphosphate
Phosphoinositide monophosphate
phosphoinositide trisphosphate
Phosphoinositides
Phosphoric Monoester Hydrolases - metabolism
PId
PIP
PIP2
PIP3
PMP
PtdIns
PtdIns(3,5)P 2
PtdIns(4,5)P 2
PtdIns4P
PtdOH
Rats
Subcellular Fractions - metabolism
thin layer chromatography
TLC
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Summary:Analyzing peroxisomal phosphoinositide (PId #) synthesis in highly purified rat liver peroxisomes we found synthesis of phosphatidylinositol 4-phosphate (PtdIns4 P), PtdIns(4,5) P 2 and PtdIns(3,5) P 2. PtdIns3 P was hardly detected in vitro, however, was observed in vivo after [ 32P]-phosphate labeling of primary rat hepatocytes. In comparison with other subcellular organelles peroxisomes revealed a unique PId pattern suggesting peroxisomal specificity of the observed synthesis. Use of phosphatase inhibitors enhanced the amount of PtdIns4 P. The results obtained provide evidence that isolated rat liver peroxisomes synthesize PIds and suggest the association of PId 4-kinase and PId 5-kinase and PId 4-phosphatase activities with the peroxisomal membrane.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.09.058