Activities of endogenous antioxidant enzymes in the cerebrospinal fluid and the hippocampus after transient forebrain ischemia in rat

Abstract The activity of SOD and CAT was measured in controls and 5 h after 5, 10 and 15 min of ischemia, as well as 1 or 2 days after 10 min of ischemia in the hippocampus and in the CSF. A significant increase in total SOD activity 5 h after ischemia was caused mainly by increased CuZn-SOD activit...

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Bibliographic Details
Published in:Journal of the neurological sciences 2007-02, Vol.253 (1), p.61-65
Main Authors: Danielisová, Viera, Gottlieb, Miroslav, Némethová, Miroslava, Burda, Jozef
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Biological and medical sciences
Catalase - cerebrospinal fluid
Cerebral ischemia
Cerebrospinal fluid
Hippocampus
Hippocampus - enzymology
Human viral diseases
Infectious diseases
Ischemic Attack, Transient - metabolism
Male
Medical sciences
Neurology
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
SOD
Superoxide Dismutase - cerebrospinal fluid
Vascular diseases and vascular malformations of the nervous system
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
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Summary:Abstract The activity of SOD and CAT was measured in controls and 5 h after 5, 10 and 15 min of ischemia, as well as 1 or 2 days after 10 min of ischemia in the hippocampus and in the CSF. A significant increase in total SOD activity 5 h after ischemia was caused mainly by increased CuZn-SOD activity. The highest values were measured 5 h after 5 min ischemia (by 160%) and smallest if 15 min (by 40%) of ischemia was used. In comparison to the hippocampus, the activity of SOD in CSF increased equally after all intervals of ischemia. Activities of total SOD and CuZn-SOD after 10 min of ischemia in the hippocampus were significantly increased only after 5 and 24 h of reperfusion but in CSF they were increased after all examined intervals of reperfusion. The activity of CAT was significantly increased in the hippocampus after 5 (by 260%), 10 and 15 min (by 100%) of ischemia. CAT activity in CSF was increased equally after all intervals of ischemia (by 200%). Ischemic attack causes a rapid response in hippocampal tissue as well as in the CSF, represented by an increase in the activity of endogenous antioxidant enzymes SOD and CAT.
ISSN:0022-510X
1878-5883
DOI:10.1016/j.jns.2006.12.001