Activities of endogenous antioxidant enzymes in the cerebrospinal fluid and the hippocampus after transient forebrain ischemia in rat

• Published in: Journal of the neurological sciences 2007-02, Vol.253 (1), p.61-65
• Main Authors: Danielisová, Viera, Gottlieb, Miroslav, Némethová, Miroslava, Burda, Jozef
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Biological and medical sciences; Catalase - cerebrospinal fluid; Cerebral ischemia; Cerebrospinal fluid; Hippocampus; Hippocampus - enzymology; Human viral diseases; Infectious diseases; Ischemic Attack, Transient - metabolism; Male; Medical sciences; Neurology; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; SOD; Superoxide Dismutase - cerebrospinal fluid; Vascular diseases and vascular malformations of the nervous system; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/j.jns.2006.12.001

Abstract The activity of SOD and CAT was measured in controls and 5 h after 5, 10 and 15 min of ischemia, as well as 1 or 2 days after 10 min of ischemia in the hippocampus and in the CSF. A significant increase in total SOD activity 5 h after ischemia was caused mainly by increased CuZn-SOD activity. The highest values were measured 5 h after 5 min ischemia (by 160%) and smallest if 15 min (by 40%) of ischemia was used. In comparison to the hippocampus, the activity of SOD in CSF increased equally after all intervals of ischemia. Activities of total SOD and CuZn-SOD after 10 min of ischemia in the hippocampus were significantly increased only after 5 and 24 h of reperfusion but in CSF they were increased after all examined intervals of reperfusion. The activity of CAT was significantly increased in the hippocampus after 5 (by 260%), 10 and 15 min (by 100%) of ischemia. CAT activity in CSF was increased equally after all intervals of ischemia (by 200%). Ischemic attack causes a rapid response in hippocampal tissue as well as in the CSF, represented by an increase in the activity of endogenous antioxidant enzymes SOD and CAT.