Constructing a novel Nanodevice powered by delta-free FoF1-ATPase

A Nanodevice was constructed by delta-free F(o)F(1)-ATPase within chromatophores and actin filaments through biotinlipid-streptavidin-biotin-(AC(5))(2)Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many delta-free F(o)F(1)-ATPase as the Nan...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-12, Vol.350 (4), p.1013-1018
Main Authors: Su, Ting, Cui, Yuanbo, Zhang, Xiaoai, Liu, Xiaolong, Yue, Jiachang, Liu, Ning, Jiang, Peidong
Format: Article
Language:English
Subjects:
Actins
Chromatophores - chemistry
Equipment Design
Equipment Failure Analysis
Linear Energy Transfer
Molecular Motor Proteins - chemistry
Motion
Nanotechnology - instrumentation
Nanotechnology - methods
Proton-Translocating ATPases - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A Nanodevice was constructed by delta-free F(o)F(1)-ATPase within chromatophores and actin filaments through biotinlipid-streptavidin-biotin-(AC(5))(2)Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many delta-free F(o)F(1)-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17-24.43mum/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating delta-free F(o)F(1)-ATPase. From bioengineering point of view, the cooperation of F(o)F(1)-ATPase is a very important research field in the future.
ISSN:0006-291X
DOI:10.1016/j.bbrc.2006.09.152