DiS-C3(3) monitoring of in vivo mitochondrial membrane potential in C. elegans

The mitochondrial respiratory chain plays a crucial role in cellular and organismal health. In addition to being the major source of energy for most cells, mitochondrial respiratory chain function regulates or modulates redox and metabolite homeostasis, apoptosis and the generation of reactive oxyge...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-03, Vol.354 (3), p.814-819
Main Authors: Gásková, Dana, DeCorby, Adrienne, Lemire, Bernard D
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Caenorhabditis elegans - physiology
Carbocyanines - pharmacology
Cell Membrane - drug effects
Cell Membrane - physiology
Electron Transport
Fluorescent Dyes
Homeostasis - physiology
Membrane Potential, Mitochondrial - drug effects
Membrane Potential, Mitochondrial - physiology
Oxidation-Reduction
Phenotype
Reactive Oxygen Species - metabolism
Spectrometry, Fluorescence
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Summary:The mitochondrial respiratory chain plays a crucial role in cellular and organismal health. In addition to being the major source of energy for most cells, mitochondrial respiratory chain function regulates or modulates redox and metabolite homeostasis, apoptosis and the generation of reactive oxygen species. In order to measure the relative in vivo mitochondrial membrane potential of different strains of the nematode, Caenorhabditis elegans, we have developed a fluorescence assay using the cationic, lipophilic carbocyanine dye, diS-C(3)(3). We demonstrate that two complex I-deficient mutants have significantly lower mitochondrial membrane potentials in vivo than wild type animals. Our fluorescence assay will enable us to better dissect and understand the complex phenotypic consequences of mitochondrial dysfunction.
ISSN:0006-291X
DOI:10.1016/j.bbrc.2007.01.073