Molecular Imaging Using a Targeted Magnetic Resonance Hyperpolarized Biosensor

A magnetic resonance approach is presented that enables high-sensitivity, high-contrast molecular imaging by exploiting xenon biosensors. These sensors link xenon atoms to specific biomolecular targets, coupling the high sensitivity of hyperpolarized nuclei with the specificity of biochemical intera...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2006-10, Vol.314 (5798), p.446-449
Main Authors: Schröder, Leif, Lowery, Thomas J, Hilty, Christian, Wemmer, David E, Pines, Alexander
Format: Article
Language:English
Subjects:
Atomic nuclei
Avidin
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Biotin
Contrast Media
Fundamental and applied biological sciences. Psychology
Imaging
Magnetic resonance
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Magnetic Resonance Spectroscopy - methods
Magnetization
Methods. Procedures. Technologies
Microspheres
Molecular imaging
Molecular structure
NMR
Nuclear magnetic resonance
Resonance
Scientific imaging
Sensitivity and Specificity
Sensors
Sepharose
Time Factors
Various methods and equipments
Xenon
Xenon Isotopes
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Summary:A magnetic resonance approach is presented that enables high-sensitivity, high-contrast molecular imaging by exploiting xenon biosensors. These sensors link xenon atoms to specific biomolecular targets, coupling the high sensitivity of hyperpolarized nuclei with the specificity of biochemical interactions. We demonstrated spatial resolution of a specific target protein in vitro at micromolar concentration, with a readout scheme that reduces the required acquisition time by >3300-fold relative to direct detection. This technique uses the signal of free hyperpolarized xenon to dramatically amplify the sensor signal via chemical exchange saturation transfer (CEST). Because it is ~10,000 times more sensitive than previous CEST methods and other molecular magnetic resonance imaging techniques, it marks a critical step toward the application of xenon biosensors as selective contrast agents in biomedical applications.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1131847