Combination of Angiotensin-Converting Enzyme and Methylenetetrahydrofolate Reductase Gene Polymorphisms as Determinant Risk Factors for Chronic Allograft Dysfunction

Abstract Objective The aim of this study was to investigate the frequency of gene angiotensin-converting enzyme insertion/deletion (ACE I/D) and methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) variants, as well as to evaluate the plasma homocysteine concentrations in 217 patients who un...

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Bibliographic Details
Published in:Transplantation proceedings 2007, Vol.39 (1), p.78-80
Main Authors: de Alvarenga, M.P.S, Pavarino-Bertelli, E.C, Abbud-Filho, M, Ferreira-Baptista, M.A.S, Haddad, R, Eberlin, M.N, Goloni-Bertollo, E.M
Format: Article
Language:English
Subjects:
Adult
Chronic Disease
Creatinine - blood
Cross-Sectional Studies
Female
Gene Deletion
Genotype
Humans
Hyperhomocysteinemia - genetics
Kidney Transplantation - adverse effects
Male
Methylenetetrahydrofolate Reductase (NADPH2) - deficiency
Methylenetetrahydrofolate Reductase (NADPH2) - genetics
Middle Aged
Peptidyl-Dipeptidase A - genetics
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Postoperative Complications - classification
Risk Factors
Surgery
Transplantation, Homologous
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Summary:Abstract Objective The aim of this study was to investigate the frequency of gene angiotensin-converting enzyme insertion/deletion (ACE I/D) and methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) variants, as well as to evaluate the plasma homocysteine concentrations in 217 patients who underwent renal transplantation at least 12 months prior to define risk factors for chronic allograft dysfunction. Methods The presence of the polymorphism ACE deletion was assessed by polymerase chain reaction (PCR) analysis. MTHFR polymorphisms were determined by PCR and restriction fragment length polymorphism (RFPL) techniques. The restriction enzymes were Hinf I and Mbo II for MTHFR variants C677T and A1298C, respectively. Plasma homocysteine concentrations were measured by liquid chromatography-tandem mass spectrometry (LS-MS/MS). Results Hyperhomocysteinemias were more common in patients with chronic allograft dysfunction ( P = .004). No statistically significant differences were observed between the allelic and genotypic distributions of MTHFR and ACE polymorphisms. An effective risk factor was found when the polymorphisms of the ACE and MTHFR genes and hyperhomocysteinemia were associated (odds ratio 2.51; 95% confidence interval 1.19–5.28). In conclusion, our study identified that the presence of hyperhomocysteinemia in combination with unfavorable genotypes contributes to an increased risk for development of chronic allograft dysfunction.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2006.10.224