A label-free protein microfluidic array for parallel immunoassays

A label‐free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein...

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Bibliographic Details
Published in:Electrophoresis 2006-10, Vol.27 (20), p.4078-4085
Main Authors: Wang, Zhan-Hui, Meng, Yong-Hong, Ying, Pei-Qing, Qi, Cai, Jin, Gang
Format: Article
Language:English
Subjects:
Animals
Hepatitis B Antibodies - analysis
Hepatitis B e Antigens - analysis
Hepatitis B Surface Antigens - analysis
Humans
Imaging ellipsometry
Immunoassay
Immunoassay - methods
Immunoglobulin G - analysis
Label-free protein microfluidic array
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Protein Array Analysis - methods
Serum Albumin - analysis
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Summary:A label‐free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label‐free format. The biological and medical applications of the label‐free protein microfluidic array are demonstrated by screening for antibody–antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200500956