A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples

Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been...

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Bibliographic Details
Published in:Parasitology 2006-11, Vol.133 (5), p.581-587
Main Authors: SANDOVAL, N., SILES-LUCAS, M., PÉREZ-ARELLANO, J. L., CARRANZA, C., PUENTE, S., LÓPEZ-ABÁN, J., MURO, A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Deoxyribonucleic acid
Design
diagnosis
DNA
DNA Primers
DNA, Helminth - urine
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Hospitals
Humans
Invertebrates
Male
Nemathelminthia. Plathelmintha
Parasites
Parasitology
Patients
PCR
Polymerase Chain Reaction - methods
Ribosomal DNA
Schistosoma - genetics
Schistosoma - isolation & purification
Schistosoma spp
Schistosomiasis
Schistosomiasis - diagnosis
Sensitivity and Specificity
Spain
Species Specificity
Tropical diseases
Urine
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Summary:Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94·4% sensitivity and 99·9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100% sensitivity and 98·9% specificity in a species-specific (S. mansoni) PCR.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182006000898