plant porphyria related to defects in plastid import of protochlorophyllide oxidoreductase A [Erratum: 2010, Mar. 23, v. 107, no. 12, p. 5693.]

The plastid envelope of higher plant chloroplasts is a focal point of plant metabolism. It is involved in numerous pathways, including tetrapyrrole biosynthesis and protein translocation. Chloroplasts need to import a large number of proteins from the cytosol because most are encoded in the nucleus....

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Published in:Proceedings of the National Academy of Sciences - PNAS 2007-02, Vol.104 (6), p.2019-2023
Main Authors: Pollmann, Stephan, Springer, Armin, Buhr, Frank, Lahroussi, Abder, Samol, Iga, Bonneville, Jean-Marc, Tichtinsky, Gabrielle, von Wettstein, Diter, Reinbothe, Christiane, Reinbothe, Steffen
Format: Article
Language:English
Subjects:
Amino acids
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis thaliana
Biochemistry, Molecular Biology
Biological Sciences
biosynthesis
Cell death
Chloroplasts
Etioplasts
Flowers & plants
Genotype & phenotype
greening
Imports
Life Sciences
Metabolism
mutants
Mutation
oxidoreductases
Oxidoreductases Acting on CH-CH Group Donors - metabolism
Pigments
plant development
Plants
Plants, Genetically Modified
Plastids
Plastids - metabolism
Porphyrias - enzymology
Porphyrias - etiology
Porphyrias - genetics
Protein precursors
protein transport
Protein Transport - physiology
protochlorophyllide oxidoreductase A
protochlorophyllides
Seedlings
Vegetal Biology
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Summary:The plastid envelope of higher plant chloroplasts is a focal point of plant metabolism. It is involved in numerous pathways, including tetrapyrrole biosynthesis and protein translocation. Chloroplasts need to import a large number of proteins from the cytosol because most are encoded in the nucleus. Here we report that a loss-of-function mutation in the outer plastid envelope 16-kDa protein (oep16) gene causes a conditional seedling lethal phenotype related to defects in import and assembly of NADPH:protochlorophyllide (Pchlide) oxidoreductase A. In the isolated knockout mutant of Arabidopsis thaliana, excess Pchlide accumulated in the dark operated as photosensitizer and provoked cell death during greening. Our results highlight the essential role of the substrate-dependent plastid import pathway of precursor Pchlide oxidoreductase A for seedling survival and the avoidance of developmentally programmed porphyria in higher plants.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0610934104