Effects of propofol and ketamine on ATP-induced contraction of the rat trachea

• Published in: Journal of anesthesia 2007-01, Vol.21 (1), p.37-41
• Main Authors: Yamaguchi, Masakazu, Shibata, Osamu, Saito, Masataka, Yoshimura, Maki, Nishioka, Kenji, Makita, Tetsuji, Sumikawa, Koji
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - administration & dosage; Analgesics - pharmacology; Anesthetics, Intravenous - pharmacology; Animals; Binding, Competitive - drug effects; Dose-Response Relationship, Drug; Ketamine - pharmacology; Male; Muscle Contraction - drug effects; Propofol - pharmacology; Rats; Rats, Wistar; Receptors, Purinergic P2 - drug effects; Receptors, Purinergic P2 - metabolism; Time Factors; Trachea - drug effects; Trachea - metabolism
• ISSN: 0913-8668; 1438-8359
• DOI: 10.1007/s00540-006-0447-0

ATP causes airway smooth-muscle contraction in patients with asthma and chronic obstructive pulmonary disease. Propofol and ketamine attenuate the airway smooth-muscle contraction induced by histamine and acetylcholine. However, it is not clear whether propofol and ketamine affect the ATP-induced airway smooth-muscle contraction. We examined the effects of propofol and ketamine on the ATP-induced contraction and ATP-P(2)-purinoceptor binding. Propofol attenuated the ATP-induced contraction in a dose-dependent manner, with a 50% inhibitory concentration of 54 +/- 22 microM. Ketamine at 300 microM attenuated the ATP-induced contraction. In the binding study, propofol attenuated the binding of the P(2)-purinoceptor with [(3)H]-ATP in a dose-dependent manner, while ketamine did not attenuate this binding. Propofol attenuates ATP-induced contraction through the inhibition of ATP-P(2)-purinoceptor binding.