Survival and differentiation of embryonic neural explants on different biomaterials

• Published in: Journal of biomedical materials research. Part A 2006-12, Vol.79A (3), p.495-502
• Main Authors: Soria, José Miguel, Martínez Ramos, Cristina, Salmerón Sánchez, Manuel, Benavent, Veronique, Campillo Fernández, Alberto, Gómez Ribelles, José Luis, García Verdugo, José Manuel, Pradas, Manuel Monleón, Barcia, Juan Antonio
• Format: Article
• Language: English
• Subjects: Animals; biocompatibility; Biocompatible Materials - metabolism; biomaterial; Cell Differentiation; Cell Movement; culture substrate; hydrogel; In Vitro Techniques; Materials Testing; Nerve Tissue - cytology; Nerve Tissue - embryology; Nerve Tissue - metabolism; neural progenitor cell; Rats; Stem Cells - cytology
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.30803

Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006