Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR

In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specifi...

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Bibliographic Details
Published in:Research in veterinary science 2007-04, Vol.82 (2), p.215-217
Main Authors: Miranda, R.R., Clara e Silva, L., Santos, H.A., Rabelo, E.M.
Format: Article
Language:English
Subjects:
Ancylostoma - genetics
Ancylostoma - isolation & purification
Ancylostoma caninum
Ancylostomiasis - parasitology
Ancylostomiasis - veterinary
Animals
circular DNA
developmental stages
Differential display
Dog Diseases - parasitology
Dogs
Female
females
gender differences
gene expression
genes
hookworms
Male
males
molecular genetics
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Helminth - chemistry
RNA, Helminth - genetics
Semi-quantitative RT-PCR
Sequence Analysis, Protein
Sex Characteristics
Sex-specific expression
sexual development
transcription (genetics)
Veterinary medicine
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Summary:In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2006.07.002