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Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR
In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specifi...
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| Published in: | Research in veterinary science 2007-04, Vol.82 (2), p.215-217 |
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| Main Authors: | , , , |
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| cites | cdi_FETCH-LOGICAL-c406t-3b4ff154bfb663d5e7c45cbdc7ae23b9e1e6ec6796156bc1c37c7e5ef8b0d0063 |
| container_end_page | 217 |
| container_issue | 2 |
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| container_title | Research in veterinary science |
| container_volume | 82 |
| creator | Miranda, R.R. Clara e Silva, L. Santos, H.A. Rabelo, E.M. |
| description | In a previous work
Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other. |
| doi_str_mv | 10.1016/j.rvsc.2006.07.002 |
| format | article |
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Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2006.07.002</identifier><identifier>PMID: 17005221</identifier><language>eng</language><publisher>England: Elsevier India Pvt Ltd</publisher><subject>Ancylostoma - genetics ; Ancylostoma - isolation & purification ; Ancylostoma caninum ; Ancylostomiasis - parasitology ; Ancylostomiasis - veterinary ; Animals ; circular DNA ; developmental stages ; Differential display ; Dog Diseases - parasitology ; Dogs ; Female ; females ; gender differences ; gene expression ; genes ; hookworms ; Male ; males ; molecular genetics ; reverse transcriptase polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA, Helminth - chemistry ; RNA, Helminth - genetics ; Semi-quantitative RT-PCR ; Sequence Analysis, Protein ; Sex Characteristics ; Sex-specific expression ; sexual development ; transcription (genetics) ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2007-04, Vol.82 (2), p.215-217</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-3b4ff154bfb663d5e7c45cbdc7ae23b9e1e6ec6796156bc1c37c7e5ef8b0d0063</citedby><cites>FETCH-LOGICAL-c406t-3b4ff154bfb663d5e7c45cbdc7ae23b9e1e6ec6796156bc1c37c7e5ef8b0d0063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17005221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miranda, R.R.</creatorcontrib><creatorcontrib>Clara e Silva, L.</creatorcontrib><creatorcontrib>Santos, H.A.</creatorcontrib><creatorcontrib>Rabelo, E.M.</creatorcontrib><title>Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>In a previous work
Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.</description><subject>Ancylostoma - genetics</subject><subject>Ancylostoma - isolation & purification</subject><subject>Ancylostoma caninum</subject><subject>Ancylostomiasis - parasitology</subject><subject>Ancylostomiasis - veterinary</subject><subject>Animals</subject><subject>circular DNA</subject><subject>developmental stages</subject><subject>Differential display</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Female</subject><subject>females</subject><subject>gender differences</subject><subject>gene expression</subject><subject>genes</subject><subject>hookworms</subject><subject>Male</subject><subject>males</subject><subject>molecular genetics</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Helminth - chemistry</subject><subject>RNA, Helminth - genetics</subject><subject>Semi-quantitative RT-PCR</subject><subject>Sequence Analysis, Protein</subject><subject>Sex Characteristics</subject><subject>Sex-specific expression</subject><subject>sexual development</subject><subject>transcription (genetics)</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kUGLFDEUhBtR3NnVP-BBGwRv3b4knWQGvCyDrsKCsu6eQzr9Ihm6k9kkPev8ezP0gOBBePAuXxVFVVW9IdASIOLjro2HZFoKIFqQLQB9Vq0IZ7ShQpDn1QqAdQ2n6_VFdZnSDgA6QuTL6oJIAE4pWVVuG7x1cdLZBV8HWyf83aQ9GmedqXPUPpno9jnVNoapvvbmOIaUw6Rro73z81TrYR5z_RTilOr-WAwm1zzO2meXi-sB67v75sf27lX1wuox4evzv6oevny-335tbr_ffNte3zamA5Eb1nfWEt71theCDRyl6bjpByM1UtZvkKBAI-RGEC56QwyTRiJHu-5hKE2wq-rD4ruP4XHGlNXkksFx1B7DnJRYbzYcunUB3_8D7sIcfcmmCDBejjBZKLpQJoaUIlq1j27S8VggdZpB7dRpBnWaQYFUZYYienu2nvsJh7-Sc-8FeLcAVgelf0WX1MNPCoQBSMEW4tNCYOnq4DCqZBx6g4OLaLIagvtfgj9uYaO1</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Miranda, R.R.</creator><creator>Clara e Silva, L.</creator><creator>Santos, H.A.</creator><creator>Rabelo, E.M.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR</title><author>Miranda, R.R. ; 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Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.</abstract><cop>England</cop><pub>Elsevier India Pvt Ltd</pub><pmid>17005221</pmid><doi>10.1016/j.rvsc.2006.07.002</doi><tpages>3</tpages></addata></record> |
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| ispartof | Research in veterinary science, 2007-04, Vol.82 (2), p.215-217 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Ancylostoma - genetics Ancylostoma - isolation & purification Ancylostoma caninum Ancylostomiasis - parasitology Ancylostomiasis - veterinary Animals circular DNA developmental stages Differential display Dog Diseases - parasitology Dogs Female females gender differences gene expression genes hookworms Male males molecular genetics reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Helminth - chemistry RNA, Helminth - genetics Semi-quantitative RT-PCR Sequence Analysis, Protein Sex Characteristics Sex-specific expression sexual development transcription (genetics) Veterinary medicine |
| title | Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR |
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