Atomic tritium as an instrument for study of protein behavior at the air–water interface

Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20 mM phosphate buffer (pH 7.0) with concentration of 2 mg/ml incubated at the room temperature for 1 h were exposed to bombardment with tritium atoms ge...

Full description

Saved in:
Bibliographic Details
Published in:Biomolecular engineering 2007-02, Vol.24 (1), p.125-129
Main Authors: Lukashina, Elena V., Badun, Gennady A., Chulichkov, Alexey L.
Format: Article
Language:English
Subjects:
Adsorption
Adsorption layers
Air
Air–water interface
Atomic tritium
Computer Simulation
Crystallography - methods
Lysozyme
Models, Chemical
Models, Molecular
Muramidase - chemistry
Muramidase - radiation effects
Muramidase - ultrastructure
Protein Conformation
Protein structure
Radiometry - methods
Surface Properties
Tritium - analysis
Tritium - chemistry
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20 mM phosphate buffer (pH 7.0) with concentration of 2 mg/ml incubated at the room temperature for 1 h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.
ISSN:1389-0344
1878-559X
DOI:10.1016/j.bioeng.2006.05.022