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Atomic tritium as an instrument for study of protein behavior at the air–water interface
Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20 mM phosphate buffer (pH 7.0) with concentration of 2 mg/ml incubated at the room temperature for 1 h were exposed to bombardment with tritium atoms ge...
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| Published in: | Biomolecular engineering 2007-02, Vol.24 (1), p.125-129 |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c360t-dc285bf740d9d5fa659fa88bf6395cb29f6140c4e78385944e0d1b969242a9a93 |
| container_end_page | 129 |
| container_issue | 1 |
| container_start_page | 125 |
| container_title | Biomolecular engineering |
| container_volume | 24 |
| creator | Lukashina, Elena V. Badun, Gennady A. Chulichkov, Alexey L. |
| description | Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20
mM phosphate buffer (pH 7.0) with concentration of 2
mg/ml incubated at the room temperature for 1
h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface. |
| doi_str_mv | 10.1016/j.bioeng.2006.05.022 |
| format | article |
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mM phosphate buffer (pH 7.0) with concentration of 2
mg/ml incubated at the room temperature for 1
h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.</description><identifier>ISSN: 1389-0344</identifier><identifier>EISSN: 1878-559X</identifier><identifier>DOI: 10.1016/j.bioeng.2006.05.022</identifier><identifier>PMID: 16870505</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adsorption ; Adsorption layers ; Air ; Air–water interface ; Atomic tritium ; Computer Simulation ; Crystallography - methods ; Lysozyme ; Models, Chemical ; Models, Molecular ; Muramidase - chemistry ; Muramidase - radiation effects ; Muramidase - ultrastructure ; Protein Conformation ; Protein structure ; Radiometry - methods ; Surface Properties ; Tritium - analysis ; Tritium - chemistry ; Water - chemistry</subject><ispartof>Biomolecular engineering, 2007-02, Vol.24 (1), p.125-129</ispartof><rights>2006 Elsevier B.V.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-dc285bf740d9d5fa659fa88bf6395cb29f6140c4e78385944e0d1b969242a9a93</citedby><cites>FETCH-LOGICAL-c360t-dc285bf740d9d5fa659fa88bf6395cb29f6140c4e78385944e0d1b969242a9a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16870505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukashina, Elena V.</creatorcontrib><creatorcontrib>Badun, Gennady A.</creatorcontrib><creatorcontrib>Chulichkov, Alexey L.</creatorcontrib><title>Atomic tritium as an instrument for study of protein behavior at the air–water interface</title><title>Biomolecular engineering</title><addtitle>Biomol Eng</addtitle><description>Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20
mM phosphate buffer (pH 7.0) with concentration of 2
mg/ml incubated at the room temperature for 1
h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.</description><subject>Adsorption</subject><subject>Adsorption layers</subject><subject>Air</subject><subject>Air–water interface</subject><subject>Atomic tritium</subject><subject>Computer Simulation</subject><subject>Crystallography - methods</subject><subject>Lysozyme</subject><subject>Models, Chemical</subject><subject>Models, Molecular</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - radiation effects</subject><subject>Muramidase - ultrastructure</subject><subject>Protein Conformation</subject><subject>Protein structure</subject><subject>Radiometry - methods</subject><subject>Surface Properties</subject><subject>Tritium - analysis</subject><subject>Tritium - chemistry</subject><subject>Water - chemistry</subject><issn>1389-0344</issn><issn>1878-559X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kM9q3DAQxkVJaZJt3yAEnXKzM7YlWboEQkiaQKCXFkovQpZHXS1reyPJG_aWd-gb9kmqZRdy62VmYL5v_vwIuaigrKAS16uy8xOOv8saQJTAS6jrD-Sskq0sOFc_T3LdSFVAw9gpOY9xBVAxYM0ncloJ2QIHfkZ-3aZp8Jam4JOfB2oiNSP1Y0xhHnBM1E2BxjT3Ozo5uglTQj_SDpdm63PHJJqWSI0Pf9_-vJqEIXtzdMbiZ_LRmXXEL8e8ID8e7r_fPRbP374-3d0-F7YRkIre1pJ3rmXQq547I7hyRsrOiUZx29XKiXy2ZdjKRnLFGEJfdUqomtVGGdUsyNVhbr7uZcaY9OCjxfXajDjNUQuplGhbloXsILRhijGg05vgBxN2ugK9Z6pX-sBU75lq4DozzbbL4_y5G7B_Nx0hZsHNQYD5y63HoKP1OFrsfUCbdD_5_2_4B_kDi3w</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Lukashina, Elena V.</creator><creator>Badun, Gennady A.</creator><creator>Chulichkov, Alexey L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070201</creationdate><title>Atomic tritium as an instrument for study of protein behavior at the air–water interface</title><author>Lukashina, Elena V. ; Badun, Gennady A. ; Chulichkov, Alexey L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-dc285bf740d9d5fa659fa88bf6395cb29f6140c4e78385944e0d1b969242a9a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adsorption</topic><topic>Adsorption layers</topic><topic>Air</topic><topic>Air–water interface</topic><topic>Atomic tritium</topic><topic>Computer Simulation</topic><topic>Crystallography - methods</topic><topic>Lysozyme</topic><topic>Models, Chemical</topic><topic>Models, Molecular</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - radiation effects</topic><topic>Muramidase - ultrastructure</topic><topic>Protein Conformation</topic><topic>Protein structure</topic><topic>Radiometry - methods</topic><topic>Surface Properties</topic><topic>Tritium - analysis</topic><topic>Tritium - chemistry</topic><topic>Water - chemistry</topic><toplevel>online_resources</toplevel><creatorcontrib>Lukashina, Elena V.</creatorcontrib><creatorcontrib>Badun, Gennady A.</creatorcontrib><creatorcontrib>Chulichkov, Alexey L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomolecular engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lukashina, Elena V.</au><au>Badun, Gennady A.</au><au>Chulichkov, Alexey L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atomic tritium as an instrument for study of protein behavior at the air–water interface</atitle><jtitle>Biomolecular engineering</jtitle><addtitle>Biomol Eng</addtitle><date>2007-02-01</date><risdate>2007</risdate><volume>24</volume><issue>1</issue><spage>125</spage><epage>129</epage><pages>125-129</pages><issn>1389-0344</issn><eissn>1878-559X</eissn><abstract>Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20
mM phosphate buffer (pH 7.0) with concentration of 2
mg/ml incubated at the room temperature for 1
h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16870505</pmid><doi>10.1016/j.bioeng.2006.05.022</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 1389-0344 |
| ispartof | Biomolecular engineering, 2007-02, Vol.24 (1), p.125-129 |
| issn | 1389-0344 1878-559X |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Adsorption Adsorption layers Air Air–water interface Atomic tritium Computer Simulation Crystallography - methods Lysozyme Models, Chemical Models, Molecular Muramidase - chemistry Muramidase - radiation effects Muramidase - ultrastructure Protein Conformation Protein structure Radiometry - methods Surface Properties Tritium - analysis Tritium - chemistry Water - chemistry |
| title | Atomic tritium as an instrument for study of protein behavior at the air–water interface |
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