In Vivo Imaging of Hepatic Growth Hormone Signaling

We developed a system to noninvasively and repeatedly image in vivo hepatic GH signaling. GH regulates postnatal growth and metabolism. It affects numerous tissues, but has major effects in liver. We used nude mice for adenoviral-mediated delivery of a signal transducer and activator of transcriptio...

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Bibliographic Details
Published in:Molecular endocrinology (Baltimore, Md.) Md.), 2006-11, Vol.20 (11), p.2819-2830
Main Authors: Frank, Stuart J, Wang, Xiangdong, He, Kai, Yang, Ning, Fang, Peng, Rosenfeld, Ron G, Hwa, Vivian, Chaudhuri, Tandra R, Deng, Luqin, Zinn, Kurt R
Format: Article
Language:English
Subjects:
Adenoviridae - metabolism
Animals
Cells, Cultured
Diagnostic Imaging - methods
Female
Gene Transfer Techniques
Genes, Reporter
Growth Hormone - metabolism
Humans
Liver - metabolism
Luciferases - metabolism
Luminescent Proteins - analysis
Male
Mice
Mice, Nude
Mice, Transgenic
Models, Biological
Receptors, Somatotropin - metabolism
Research Design
Signal Transduction
STAT5 Transcription Factor - metabolism
Transcriptional Activation
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Summary:We developed a system to noninvasively and repeatedly image in vivo hepatic GH signaling. GH regulates postnatal growth and metabolism. It affects numerous tissues, but has major effects in liver. We used nude mice for adenoviral-mediated delivery of a signal transducer and activator of transcription 5-dependent GH response element, a luciferase reporter to detect GH signaling pathway activation. We detected by noninvasive bioluminescence imaging GH-induced hepatic GH signaling serially within intact mice. Statistically significant effects of GH dose and time dependence were detected in the liver luciferase signal that peaked 3 h after GH injection. Codelivery of GH receptor significantly enhanced GH response, an effect that was further augmented by fasting. Our imaging system allows detailed in vivo analysis of GH signaling and action and may be a paradigm for studies of additional signaling pathways in liver and other tissues.
ISSN:0888-8809
1944-9917
DOI:10.1210/me.2005-0532