Isolation and characterization of chitin from bumblebee ( Bombus terrestris)

Insect chitin possessing shell-like structure was prepared from the bumblebee corpses by a consequent treatment with 1 M HCl and 1 M NaOH. The bumblebee chitin was compared with crustacean (shrimp) chitin by using elemental analysis, Fourier-transform infrared (FT-IR) and solid-state 13C cross-polar...

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Bibliographic Details
Published in:International journal of biological macromolecules 2007-02, Vol.40 (3), p.237-241
Main Authors: Majtán, Juraj, Bíliková, Katarína, Markovič, Oskar, Gróf, Ján, Kogan, Grigorij, Šimúth, Jozef
Format: Article
Language:English
Subjects:
Acetylation
Animals
Bombus terrestris
Bumblebee
Chitin
Chitin - chemistry
Chitin - isolation & purification
Decapoda (Crustacea) - chemistry
Hymenoptera - chemistry
Microscopy
Nuclear Magnetic Resonance, Biomolecular
Shrimp
Spectroscopy
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Summary:Insect chitin possessing shell-like structure was prepared from the bumblebee corpses by a consequent treatment with 1 M HCl and 1 M NaOH. The bumblebee chitin was compared with crustacean (shrimp) chitin by using elemental analysis, Fourier-transform infrared (FT-IR) and solid-state 13C cross-polarization magic angle spinning nuclear magnetic resonance (CP/MAS)-NMR spectroscopy and confocal microscopy. Both chitins (bumblebee and shrimp) exhibited identical spectra, while the bumblebee chitin had a 5% lower degree of acetylation and was characterized by a fine membrane texture.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2006.07.010