CRP determination based on a novel magnetic biosensor

The c-reactive protein (CRP) is a very significant human blood marker for inflammatory processes and is routinely determined for many clinical purposes. The widespread and well established detection method for this ∼115 kDa hepatic protein is the high-sensitivity ELISA assay (hsCRP-ELISA) in blood s...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2007-01, Vol.22 (6), p.973-979
Main Authors: Meyer, Martin H.F., Hartmann, Markus, Krause, Hans-Joachim, Blankenstein, Gert, Mueller-Chorus, Birgit, Oster, Juergen, Miethe, Peter, Keusgen, Michael
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers - blood
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Blood Chemical Analysis - instrumentation
Blood Chemical Analysis - methods
c-reactive protein (CRP)
C-Reactive Protein - analysis
CVD
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Humans
IBD
Immunoassay - instrumentation
Immunoassay - methods
Immunomagnetic Separation - instrumentation
Immunomagnetic Separation - methods
Immunosensor
Inflammation
Inflammation - blood
Inflammation - diagnosis
Magnetic detection
Magnetics - instrumentation
Methods. Procedures. Technologies
Microchemistry - instrumentation
Microchemistry - methods
Sensitivity and Specificity
Various methods and equipments
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Description
Summary:The c-reactive protein (CRP) is a very significant human blood marker for inflammatory processes and is routinely determined for many clinical purposes. The widespread and well established detection method for this ∼115 kDa hepatic protein is the high-sensitivity ELISA assay (hsCRP-ELISA) in blood serum. New approaches in medical CRP diagnosis (e.g. for CVD, inflammatory bowel disease) require rapid quantification in native matrices. A novel CRP determination method based on magnetic detection is described and tested for human blood serum, saliva and urine. The detection principle is based on two different anti-CRP antibodies (monoclonal, IgG) for CRP trapment and labelling. The linear detection range of this immunosensor ranged from 25 ng/ml to 2.5 μg/ml and is therefore much more sensitive than typical hsCRP-ELISA-assays.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2006.04.001