Involvement of membrane protein GDE2 in retinoic acid-induced neurite formation in Neuro2A cells

We show that a glycerophosphodiester phosphodiesterase homolog, GDE2, is widely expressed in brain tissues including primary neurons, and that the expression of GDE2 in neuroblastoma Neuro2A cells is significantly upregulated during neuronal differentiation by retinoic acid (RA) treatment. Stable ex...

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Bibliographic Details
Published in:FEBS letters 2007-02, Vol.581 (4), p.712-718
Main Authors: Yanaka, Noriyuki, Nogusa, Yoshihito, Fujioka, Yuichiro, Yamashita, Yosuke, Kato, Norihisa
Format: Article
Language:English
Subjects:
Animals
Brain - drug effects
Brain - enzymology
Brain - metabolism
DMEM
Dulbecco's modified Eagle's medium
Enzyme Activation - drug effects
Extracellular Signal-Regulated MAP Kinases - metabolism
FCS
fetal calf serum
GDE2
Gene Expression - drug effects
Gene Expression Profiling
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Neoplastic - drug effects
GFP
Glycerophosphodiester phosphodiesterase
GP-PDE
green fluorescent protein
Humans
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Neurites - drug effects
Neurites - enzymology
Neuro2A cells
Neuroblastoma - enzymology
Neuroblastoma - pathology
Phosphoric Diester Hydrolases - genetics
Phosphoric Diester Hydrolases - metabolism
Protein Transport - drug effects
Retinoic acid
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - metabolism
Tretinoin - pharmacology
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Summary:We show that a glycerophosphodiester phosphodiesterase homolog, GDE2, is widely expressed in brain tissues including primary neurons, and that the expression of GDE2 in neuroblastoma Neuro2A cells is significantly upregulated during neuronal differentiation by retinoic acid (RA) treatment. Stable expression of GDE2 resulted in neurite formation in the absence of RA, and GDE2 accumulated at the regions of perinuclear and growth cones in Neuro2A cells. Furthermore, a loss-of-function of GDE2 in Neuro2A cells by RNAi blocked RA-induced neurite formation. These results demonstrate that GDE2 expression during neuronal differentiation plays an important role for growing neurites.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2007.01.035