Variation in the content of hypericins in four generations of seed progeny of Hypericum perforatum somaclones

The content of hypericins in in vitro regenerated Hypericum perforatum plants (Rsub(0)) and four generations of their seed progeny (Rsub(1)-Rsub(4)) was compared. The mean content of hypericins in fieldgrown plants over the period 1992-2002 gradually increased under selection, and in the Rsub(4) gen...

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Bibliographic Details
Published in:Journal of plant research 2007-01, Vol.120 (1), p.123-128
Main Authors: Koperdakova, J.(P.J. Safarik Univ., Kosice (Slovakia)), Kosuth, J, Cellarova, E
Format: Article
Language:English
Subjects:
APOMIXIE
APOMIXIS
Base Sequence
CHEMICAL COMPOSITION
Chromosomes, Plant
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DNA Primers
DNA, Plant - genetics
Environmental conditions
Genetic Variation
Genomics
Growing season
Hypericum - embryology
Hypericum - genetics
Hypericum - metabolism
HYPERICUM PERFORATUM
METABOLITE SECONDAIRE
METABOLITOS SECUNDARIOS
Minisatellite Repeats
Perylene - analogs & derivatives
Perylene - metabolism
Plant biology
Plant reproduction
POLIPLOIDIA
Polymerase Chain Reaction
POLYPLOIDIE
POLYPLOIDY
SECONDARY METABOLITES
Seeds
Seeds - metabolism
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Summary:The content of hypericins in in vitro regenerated Hypericum perforatum plants (Rsub(0)) and four generations of their seed progeny (Rsub(1)-Rsub(4)) was compared. The mean content of hypericins in fieldgrown plants over the period 1992-2002 gradually increased under selection, and in the Rsub(4) generation it was almost seven-times higher than that in the Rsub(0) soma-clones. Significant difference between hypericin content in diploids and tetraploids was detected in Rsub(0), Rsub(1) and Rsub(3) generations. Hypericin content in four diploid and tetraploid lineages originated from a single soma-clone was genotype dependent. To eliminate the influence of environmental conditions during different growing seasons, we used seeds of selected Rsub(0)-Rsub(3) plants to derive R'sub(1) to R'sub(4) generations cultivated during the same years. In this case no statistically significant difference in hypericin content was found between the R'sub(1)-R'sub(4) generations. Apomictically and sexually derived plants were distinguished by PCR using variable numbers of tandem repeats (VNTR) primers. The content of hypericins in apomictically derived progenies was compared.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-006-0031-6