New chromogenic and fluorogenic reagents and sensors for neutral and ionic analytes based on covalent bond formation-a review of recent developments

• Published in: Analytical and bioanalytical chemistry 2006-11, Vol.386 (5), p.1201-1214
• Main Author: Mohr, Gerhard J
• Format: Article
• Language: English
• Subjects: Amines; Amino acids; Aptamers; Biomolecules; Carbohydrates; Chemical bonds; Chemical sensors; Chemical warfare agents; Chemoreceptors; Chemosensor; Covalence; Covalent bonds; Cyanides; Diamines; Hydrogen bonding; Hydrogen peroxide; Hydrophobicity; Ionophores; Nitrogen oxides; Optical communication; Optical probe; Optical sensor; Organophosphates; Peptides; Photochemicals; Reagents; Recognition; Saccharides; Selectivity; Signal transduction
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-006-0647-3

To date, hydrogen bonding and Coulomb, van der Waals and hydrophobic interactions are the major contributors to non-covalent analyte recognition using ionophores, ligands, aptamers and chemosensors. However, this article describes recent developments in the use of (reversible) covalent bond formation to detect analyte molecules, with special focus on optical signal transduction. Several new indicator dyes for analytes such as amines and diamines, amino acids, cyanide, formaldehyde, hydrogen peroxide, organophosphates, nitrogen oxide and nitrite, peptides and proteins, as well as saccharides have become available. New means of converting analyte recognition into optical signals have also been introduced, such as colour changes of chiral nematic layers. This article gives an overview of recent developments and discusses response mechanisms, selectivity and sensitivity.