Effects of Cyclic Hydrostatic Pressure on the Metabolism of Human Osteoarthritic Chondrocytes Cultivated in a Collagen Gel

:  Among other parameters, the application of mechanical force may provide an important stimulus in modulating the structure and function of tissue‐engineered articular cartilage. We developed a cultivation chamber in which six collagen type‐I gel samples, seeded with human osteoarthritic chondrocyt...

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Bibliographic Details
Published in:Artificial organs 2007-02, Vol.31 (2), p.91-98
Main Authors: Gavénis, Karsten, Kremer, André, Von Walter, Matthias, Hollander, Dirk A., Schneider, Ulrich, Schmidt-Rohlfing, Bernhard
Format: Article
Language:English
Subjects:
Aged
Aggrecans - metabolism
Cartilage, Articular - cytology
Cells, Cultured
Chondrocytes - metabolism
Collagen gel
Collagen Type II - metabolism
Cultivation chamber
Cyclic hydrostatic pressure
Female
Gels
Gene expression
Human osteoarthritic chondrocytes
Humans
Hydrostatic Pressure
Immunohistochemistry
Male
Osteoarthritis - metabolism
Proteoglycans - analysis
RNA, Messenger - analysis
Spectrophotometry
Tissue Engineering - methods
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Summary::  Among other parameters, the application of mechanical force may provide an important stimulus in modulating the structure and function of tissue‐engineered articular cartilage. We developed a cultivation chamber in which six collagen type‐I gel samples, seeded with human osteoarthritic chondrocytes, can be cultivated simultaneously. A cyclic hydrostatic pressure of up to 40 kPa with a frequency of 0.0125 Hz was applied, and cultivation was performed for 1, 4, 7, or 14 days. Histological examinations revealed a spheroidal cell morphology in the treatment group. In contrast, control samples of the same patients represented a more fibroblastic appearance. Collagen type‐II (col‐II) protein was found in the very pericellular region of all investigated samples; the col‐II content did not obviously vary between the control and treatment groups. In the treatment group, col‐II and aggrecan gene expression were elevated. A spectrophotometric quantification of proteoglycan concentrations in media supernatants revealed a statistically significant enhancement in the treatment group.
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2007.00347.x