First Application of a Microsphere-Based Immunoassay to the Detection of Genetically Modified Organisms (GMOs):  Quantification of Cry1Ab Protein in Genetically Modified Maize

An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, a specific protocol was developed to a...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-02, Vol.55 (4), p.1071-1076
Main Authors: Fantozzi, Anna, Ermolli, Monica, Marini, Massimiliano, Scotti, Domenico, Balla, Branko, Querci, Maddalena, Langrell, Stephen R. H, Van den Eede, Guy
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacterial Toxins - analysis
Bacterial Toxins - genetics
Biological and medical sciences
Cereal and baking product industries
Endotoxins - analysis
Endotoxins - genetics
Fluorescent Dyes
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Hemolysin Proteins - analysis
Hemolysin Proteins - genetics
Immunoassay - methods
Methods of analysis, processing and quality control, regulation, standards
Microspheres
Plants, Genetically Modified - chemistry
Plants, Genetically Modified - genetics
Seeds - chemistry
Zea mays
Zea mays - chemistry
Zea mays - genetics
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Summary:An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, a specific protocol was developed to assess the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% [weight of genetically modified organism (GMO)/weight[. Test linearity was achieved in the range of values from 0.1 to 3%, whereas fluorescence signal increased following a nonlinear model, reaching a plateau at 25%. The limits of detection and quantification were equal to 0.018 and 0.054%, respectively. The present study describes the first application of quantitative high-throughput immunoassays in GMO analysis. Keywords: GMO; immunoassay; Cry1Ab; high-throughput analysis; quantitative detection assay; fluorescent beads
ISSN:0021-8561
1520-5118
DOI:10.1021/jf061506p