Study of normal colorectal tissue by FT-Raman spectroscopy

FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected from 39 patients, providing 144 spectra for the stati...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2007-03, Vol.387 (5), p.1643-1648
Main Authors: Andrade, P. O, Bitar, R. A, Yassoyama, K, Martinho, H, Santo, A. M. E, Bruno, P. M, Martin, A. A
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers - analysis
Biomarkers - chemistry
Colon - chemistry
Colorectal
Female
FT-Raman spectroscopy
Humans
Male
Middle Aged
neoplasms
Optical biopsy
Rectum - chemistry
Reference Values
Spectroscopy, Fourier Transform Infrared - methods
Spectrum Analysis, Raman - methods
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Summary:FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected from 39 patients, providing 144 spectra for the statistical analysis. The results enable one to estabilish three well-defined spectroscopic groups of non-altered coloretal tissues that were consistently checked by statistical (clustering) and biological (histopathology) analyses: group 1 is represented by samples with the presence of epithelial layer, connective tissue papillae, and smooth muscle tissue; group 2 comprises tissues with epithelial layer and connective tissue papillae; group 3 presented mostly fatty and slack conjunctive tissue. The study reveals the existence of an intrinsic spectral variability for each patient that must be considered when sampling tissues fragments to build a spectral database. This is the first step for future studies and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancer.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-0819-1