Differential expression of two flavonoid 3'-hydroxylase cDNAs involved in biosynthesis of anthocyanin pigments and 3-deoxyanthocyanidin phytoalexins in sorghum [Sorghum bicolor]

Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H...

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Bibliographic Details
Published in:Plant and cell physiology 2006-10, Vol.47 (10), p.1412-1419
Main Authors: Shin, C.H.(University of Hong Kong (China)), Chu, I.K, Yip, W.K, Lo, C
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
ANTHOCYANE
ANTHOCYANINS
Anthocyanins - biosynthesis
Anthocyanins - chemistry
ANTOCIANINAS
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
DNA
DNA, Complementary - analysis
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes, Plant
HYDROXYLATION
IDROXILACION
Mixed Function Oxygenases - biosynthesis
Mixed Function Oxygenases - genetics
Molecular Sequence Data
Molecular Structure
Sesquiterpenes
Sorghum - genetics
SORGHUM BICOLOR
Terpenes - chemistry
Terpenes - metabolism
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Summary:Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylated flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcl003