Distribution of CCR3 mRNA expression in horse tissues

CCL11 (also known as eotaxin) is a very potent and selective mediator of eosinophil migration which exerts its effects through its receptor, CCR3. In this study we report the cloning of an equine CCR3 cDNA sequence and investigation of the localization of CCR3 mRNA expression in horse tissues. Equin...

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Bibliographic Details
Published in:Veterinary Immunology and Immunopathology 2006-12, Vol.114 (3), p.238-246
Main Authors: Weston, Marie C., Cunningham, Fiona M., Collins, Margaret E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
CCL11
Chemokine receptor
Cloning, Molecular
Colon - immunology
Eosinophils - immunology
Eotaxin
Equine CCR3
Horses - genetics
Horses - immunology
In Situ Hybridization - veterinary
Lung - immunology
Molecular Sequence Data
Receptors, CCR3
Receptors, Chemokine - biosynthesis
Receptors, Chemokine - genetics
Receptors, Chemokine - immunology
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Sequence Analysis, DNA
Spleen - immunology
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Summary:CCL11 (also known as eotaxin) is a very potent and selective mediator of eosinophil migration which exerts its effects through its receptor, CCR3. In this study we report the cloning of an equine CCR3 cDNA sequence and investigation of the localization of CCR3 mRNA expression in horse tissues. Equine CCR3 displayed high levels of sequence identity with CCR3 sequences in other species. RT-PCR analysis revealed the expression of CCR3 in colon, lung and spleen of normal horses. In situ hybridisation experiments indicated that expression of CCR3 mRNA in colon was predominantly in eosinophils and to a lesser extent in mast cells, whereas CCR3 was seen mainly in lymphocytes of the lung and spleen. In view of the role of CCR3 in the recruitment of cells into sites of allergic inflammation, equine-specific CCR3 sequence data and information on tissue localization will be of potential benefit in the development of CCR3-targeted anti-inflammatory therapies in the horse.
ISSN:0165-2427
1873-2534
1365-2567
DOI:10.1016/j.vetimm.2006.08.010