A simple method to obtain en face sections of flat tissue culture cells

A method is described for embedment of multiple confluent sheets of flat tissue culture cells that permits sectioning for thin or semithin sections in precise planes. The technique is especially useful for obtaining en face sections. Microsc. Res. Tech., 2006. © 2006 Wiley‐Liss, Inc.

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Bibliographic Details
Published in:Microscopy research and technique 2006-11, Vol.69 (11), p.924-926
Main Authors: Piras, Monica, Diaz, Giacomo, Tandler, Bernard, Bernardini, Silvana, Cossu, Margherita, Lantini, Maria S., Falchi, Angela M., Melis, Marta, Piludu, Marco
Format: Article
Language:English
Subjects:
Cells, Cultured
Microscopy
Microscopy, Electron, Transmission
Microtomy - methods
monolayer culture cells
Tissue Embedding - methods
transmission electron microscopy
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Description
Summary:A method is described for embedment of multiple confluent sheets of flat tissue culture cells that permits sectioning for thin or semithin sections in precise planes. The technique is especially useful for obtaining en face sections. Microsc. Res. Tech., 2006. © 2006 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.20368