The complete nucleotide sequence of the mitochondrial genome of Tetraodon nigroviridis

The fresh water pufferfish Tetraodon nigroviridis is a model organism for studying evolution of genome and gene functions, but its mitochondrial genome (mtDNA) sequence is still not available. We determined the complete nucleotide sequence of its mtDNA using shotgun sequencing. The T. nigroviridis m...

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Bibliographic Details
Published in:DNA sequence 2006-04, Vol.17 (2), p.115-121
Main Authors: Yue, Gen Hua, Lo, Loong Chueng, Zhu, Ze Yuan, Lin, Grace, Feng, Felicia
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Evolution
DNA, Mitochondrial - genetics
Freshwater
Fugu rubripes
Genes, Mitochondrial
Locus Control Region - genetics
Molecular Sequence Data
Phylogeny
Polymorphism, Single Nucleotide
Sequence Alignment
Tetraodon
Tetraodon nigroviridis
Tetraodontiformes - classification
Tetraodontiformes - genetics
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Summary:The fresh water pufferfish Tetraodon nigroviridis is a model organism for studying evolution of genome and gene functions, but its mitochondrial genome (mtDNA) sequence is still not available. We determined the complete nucleotide sequence of its mtDNA using shotgun sequencing. The T. nigroviridis mtDNA was 16,462 bp, and contained 13 protein coding genes, 22 tRNAs, 2 rRNAs and a major non-coding region. The gene order was identical to the common type of vertebrate mtDNA, whereas the G+C content in the sense strand was 46.9%, much higher than most other fish species. One hundred and three SNPs were detected in the control region of the mtDNA of 35 individuals, a majority of SNPs were detected in the 5′ end of the control region. A phylogenetic study including 21 fish species was performed on concatenated amino acid sequences of 12 protein coding genes, and revealed that the T. nigroviridis was clustered with Fugu rubripes into a group. The complete mtDNA sequence and SNPs in its control region will be useful in studying fish evolution, in differentiating different Tetraodon species and in analyzing genetic diversity within T. nigroviridis.
ISSN:1042-5179
1029-2365
DOI:10.1080/10425170600700378