Capillary Isoelectric Focusing — Useful tool for detection of the biofilm formation in Staphylococcus epidermidis

The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The p...

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Bibliographic Details
Published in:Journal of microbiological methods 2007-03, Vol.68 (3), p.530-535
Main Authors: Ruzicka, Filip, Horka, Marie, Hola, Veronika, Votava, Miroslav
Format: Article
Language:English
Subjects:
Amidohydrolases - genetics
Bacterial Proteins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biofilm
Biofilm detection
Biofilms - growth & development
Biological and medical sciences
Capillary Isoelectric Focusing
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Fundamental and applied biological sciences. Psychology
Humans
Isoelectric Focusing - instrumentation
Isoelectric Focusing - methods
Isoelectric Point
Microbiology
Operon
Polysaccharides, Bacterial - metabolism
Slime
Staphylococcus epidermidis
Staphylococcus epidermidis - genetics
Staphylococcus epidermidis - growth & development
Staphylococcus epidermidis - isolation & purification
Ultrasonics
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Summary:The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73 S. epidermidis strains were examined for the presence of ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (p I) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near p I value 2.3, while the p I values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative S. epidermidis strains.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2006.10.008