Methods for building an olfactometer with known concentration outcomes

We provide detailed instructions and part selections for construction of a five-channel air dilution olfactometer capable of generating neat odorants and binary mixtures at a range of known concentrations. At the heart of the olfactometer is an odorant canister that is (1) cheap and readily availabl...

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Bibliographic Details
Published in:Journal of neuroscience methods 2007-03, Vol.160 (2), p.231-245
Main Authors: Johnson, Bradley N., Sobel, Noam
Format: Article
Language:English
Subjects:
Atmosphere Exposure Chambers - standards
Atmosphere Exposure Chambers - trends
Biological Assay - instrumentation
Biological Assay - methods
Electronics, Medical - instrumentation
Electronics, Medical - methods
Equipment and Supplies - standards
fMRI
Gases - analysis
Humans
Magnetic Resonance Imaging
Methods
Neurophysiology - instrumentation
Neurophysiology - methods
Odorants - analysis
Olfaction
Olfactometer
Psychophysics
Psychophysics - instrumentation
Psychophysics - methods
Smell - physiology
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Summary:We provide detailed instructions and part selections for construction of a five-channel air dilution olfactometer capable of generating neat odorants and binary mixtures at a range of known concentrations. At the heart of the olfactometer is an odorant canister that is (1) cheap and readily available, (2) safe and durable, (3) has minimal odor adherence, (4) is easily incorporated into any olfactometer, and critically (5) produces a highly consistent stimulus. By flowing a given carrier gas at a given flowrate through a given odorant in this canister, the same end-vapor is achieved. Flow/concentration outcomes are provided for several odorants routinely used in olfactometry. This tool will enable researchers to generate known concentrations without expensive analytical machinery.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2006.09.008