Functional Polymorphisms in the Matrix Metalloproteinase‐9 Gene in Relation to Severity of Chronic Periodontitis

• Published in: Journal of periodontology (1970) 2006-11, Vol.77 (11), p.1850-1855
• Main Authors: Holla, Lydie Izakovicova, Fassmann, Antonin, Mužík, Jan, Vanek, Jiri, Vasku, Anna
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Case-Control Studies; Chronic Disease; Cytosine; Czech Republic; Dentistry; Female; Gene Frequency; Genes; Genetic Predisposition to Disease; Humans; Male; Matrix Metalloproteinase 9 - genetics; matrix metalloproteinases; Middle Aged; periodontitis; Periodontitis - enzymology; Periodontitis - genetics; polymorphism; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; sex characteristics; Sex Factors; Thymine
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2006.050347

Background: Members of the matrix metalloproteinase (MMP) family are implicated in the chronic remodeling in periodontal diseases. Therefore, we performed a case‐control study to investigate a plausible association between susceptibility to chronic periodontitis (CP) and the polymorphisms in the MMP‐9 (gelatinase B) gene. Methods: Using polymerase chain reaction with subsequent restriction analyses, MMP‐9 −1562C/T and R+279Q variants were determined in 304 subjects (169 patients with CP, 76 with a mild to moderate form and 93 with severe generalized CP, and 135 age‐ and gender‐matched unrelated control subjects). Results: The distribution of the MMP‐9 −1562C/T and R+279Q genotypes and alleles did not significantly differ between cases and controls. However, the frequency of the T variant at position −1562 was marginally higher in patients with severe disease compared to those with mild to moderate forms (P 0.05). In further analysis, the −1562T allele was associated with a severe form of CP in men (odds ratio: 3.87; 95% confidence interval: 1.40 to 10.65; P