Effect of two different approaches for root decontamination on new cementum formation following guided tissue regeneration: a histomorphometric study in dogs

• Published in: Journal of periodontal research 2006-12, Vol.41 (6), p.535-540
• Main Authors: Gonçalves, P. F., Gurgel, B. C. V., Pimentel, S. P., Sallum, E. A., Sallum, A. W., Casati, M. Z., Nociti Jr, F. H.
• Format: Article
• Language: English
• Subjects: Animals; Biofilms; Biological and medical sciences; Decontamination - methods; Dental Cementum - cytology; Dental Cementum - microbiology; Dental Cementum - physiology; Dental Polishing; Dental Scaling; Dentistry; Dogs; Furcation Defects - microbiology; Furcation Defects - pathology; Furcation Defects - therapy; Guided Tissue Regeneration, Periodontal - methods; Ligation; Male; Medical sciences; Otorhinolaryngology. Stomatology; periodontal regeneration; Random Allocation; root cementum; Root Planing; Tooth Root - cytology; Tooth Root - microbiology
• ISSN: 0022-3484; 1600-0765
• DOI: 10.1111/j.1600-0765.2006.00902.x

Background and Objective:  The aim of the present study was to evaluate comparatively the effect of two different approaches for root decontamination on new cementum formation following guided tissue regeneration (GTR). Material and Methods:  Nine mongrel dogs were used to obtain bilateral chronic class III furcation defects by placing cotton ligatures around both third mandibular premolars. The teeth were randomly assigned to receive one of the following treatments: scaling and root planing, by means of hand and rotatory instruments, in order to remove soft and hard deposits as well as all root cementum (group A); or removal of only soft microbial deposits, by polishing the root surface with rubber cups and polishing paste, aiming for maximum root cementum preservation (group B). Both groups were treated with GTR, with the use of resorbable polyglycolic‐lactic acid membranes (RESOLUT XT®). Results:  Four months later, data analysis showed that a superior length (mm) (3.59 ± 1.67 and 6.20 ± 2.26 for groups A and B, respectively; p = 0.004) and a thicker layer (µm) (18.89 ± 9.47 and 52.29 ± 22.48 for groups A and B, respectively; p = 0.001) of new cementum was achieved by keeping the root cementum in place during root decontamination (group B). Regardless of the treatment modality, the new cementum was predominantly of a reparative, cellular extrinsic and intrinsic fiber type. Conclusion:  Within the limits of the present study, it may be concluded that root cementum preservation may affect the new cementum formation following GTR in class III furcation defects, and the treatment modality did not influence the type of newly formed cementum.