Location of putative stem cells in human periodontal ligament

Background and Objective:  The origin of cells in the mature periodontium, and the location of their progenitors, are still unknown. It is also unknown whether inflammation influences the number and distribution of these cells within the periodontium. Molecules such as STRO‐1, CD146 and CD44 have be...

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Bibliographic Details
Published in:Journal of periodontal research 2006-12, Vol.41 (6), p.547-553
Main Authors: Chen, S. C., Marino, V., Gronthos, S., Bartold, P. M.
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - analysis
Biological and medical sciences
CD146 Antigen - analysis
cementum
Dentistry
Humans
Hyaluronan Receptors - analysis
Medical sciences
Mice
Otorhinolaryngology. Stomatology
periodontal ligament
Periodontal Ligament - chemistry
Periodontal Ligament - cytology
Periodontitis - immunology
Periodontitis - pathology
stem cells
Stem Cells - cytology
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Summary:Background and Objective:  The origin of cells in the mature periodontium, and the location of their progenitors, are still unknown. It is also unknown whether inflammation influences the number and distribution of these cells within the periodontium. Molecules such as STRO‐1, CD146 and CD44 have been identified on a variety of mesenchymal stem cells. The aim of this study was to identify and localize putative stem cells in diseased and healthy human periodontal ligament using cell‐surface markers for mesenchymal stem cells. Material and Methods:  Healthy and periodontitis‐affected teeth were collected, fixed in 10% neutral‐buffered formalin, decalcified and embedded in paraffin in preparation for immunohistochemistry. Antibodies against STRO‐1, CD146 and CD44 were used to identify putative stem cells in the periodontal ligament. Results:  Putative stem cells were identified in both healthy and diseased periodontal ligament. They were mainly located in the paravascular region and small clusters of cells were also found in the extravascular region. Wider distributions of these cells were detected in sections of diseased ligament. Conclusion:  Within the periodontal ligament of both healthy and diseased teeth, cells have been identified consistent with their identification as putative stem cells. The presence of an inflammatory reaction associated with periodontitis may enhance the number of these cells.
ISSN:0022-3484
1600-0765
DOI:10.1111/j.1600-0765.2006.00904.x