Growth and metabolism in sugarcane are altered by the creation of a new hexose-phosphate sink

• Published in: Plant biotechnology journal 2007-03, Vol.5 (2), p.240-253
• Main Authors: Chong, Barrie Fong, Bonnett, Graham D, Glassop, Donna, O'Shea, Michael G, Brumbley, Stevens M
• Format: Article
• Language: English
• Subjects: glucose-6-phosphate; Hexosephosphates - metabolism; Metabolic Networks and Pathways; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - metabolism; Saccharum; Saccharum - enzymology; Saccharum - genetics; Saccharum - growth & development; Saccharum - metabolism; Saccharum spp; sorbitol; Sorbitol - metabolism; sorbitol-6-phosphate dehydrogenase; Sucrose - metabolism
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/j.1467-7652.2006.00235.x

An efficient in planta sugarcane-based production system may be realized by coupling the synthesis of alternative products to the metabolic intermediates of sucrose metabolism, thus taking advantage of the sucrose-producing capability of the plant. This was evaluated by synthesizing sorbitol in sugarcane (Saccharum hybrids) using the Malus domestica sorbitol-6-phosphate dehydrogenase gene (mds6pdh). Mature transgenic sugarcane plants were compared with untransformed sugarcane variety Q117 by evaluation of the growth, metabolite levels and extractable activity of relevant enzymes. The average amounts of sorbitol detected in the most productive line were 120 mg/g dry weight (equivalent to 61% of the soluble sugars) in the leaf lamina and 10 mg/g dry weight in the stalk pith. The levels of enzymes involved in sucrose synthesis and cleavage were elevated in the leaves of plants accumulating sorbitol, but this did not affect sucrose accumulation in the culm. The activity of oxidative reactions in the pentose phosphate pathway and the non-reversible glyceraldehyde-3-phosphate dehydrogenase reaction were elevated to replenish the reducing power consumed by sorbitol synthesis. Sorbitol-producing sugarcane generated 30%-40% less aerial biomass and was 10%-30% shorter than control lines. Leaves developed necrosis in a pattern characteristic of early senescence, and the severity was related to the relative quantity of sorbitol accumulated. When the Zymomonas mobilis glucokinase (zmglk) gene was co-expressed with mds6pdh to increase the production of glucose-6-phosphate, the plants were again smaller, indicating that glucose-6-phosphate deficiency was not responsible for the reduced growth. In summary, sorbitol hyperaccumulation affected sugarcane growth and metabolism, but the outcome was not lethal for the plant. This work also demonstrated that impressive yields of alternative products can be generated from the intermediates of sucrose metabolism in Saccharum spp.