Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9

We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 cou...

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Bibliographic Details
Published in:FEBS letters 2007-12, Vol.581 (29), p.5649-5657
Main Authors: Arai, Shigeki, Matsushita, Akio, Du, Kun, Yagi, Ken, Okazaki, Yasushi, Kurokawa, Riki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Apoptosis
BAX
bcl-2-Associated X Protein - genetics
bcl-2-Associated X Protein - metabolism
BCL2-associated X protein
Catalysis
Cell Differentiation
Cell Proliferation
Cloning, Molecular
DNA, Complementary - metabolism
GAPDH
GFP
glyceraldehyde-3-phosphate dehydrogenase
green fluorescent protein
HIPK
homeodomain-interacting protein kinase
Humans
Inhibitor of Apoptosis Proteins
Kinase
MBP
MDM2
Mice
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Molecular Sequence Data
mouse double minute 2 homolog
myelin basic protein
national center for biotechnology information
NCBI
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
p53
Promoter Regions, Genetic
Protein-Serine-Threonine Kinases - analysis
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Sequence Alignment
Serine - genetics
Serine - metabolism
Transcription
Tumor suppressor
Tumor Suppressor Protein p53 - metabolism
ultraviolet
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Summary:We describe here the cloning and characterization of a novel mouse homeodomain-interacting protein kinase (HIPK)-like gene, Hipk4. Hipk4 is expressed in lung and in white adipose tissue and encodes a 616 amino acid protein that includes a serine/threonine kinase domain. We demonstrate that HIPK4 could phosphorylate human p53 protein at serine 9, both in vitro and in vivo. Among known p53-responsive promoters, activity of the human survivin promoter, which is repressed by p53, was decreased by HIPK4 in p53 functional A549 cells. Human BCL2-associated X protein-promoter activity was not affected. These findings suggest that phosphorylation of p53 at serine 9 is important for p53 mediated transcriptional repression.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2007.11.022