Loading…
Efficient capture of cardiogenesis-associated genes expressed in ES cells
Cardiogenesis can be induced in vitro in ES cells, though it is difficult to distinguish cardiac-specific genes, since embryoid bodies simultaneously differentiate into multiple lineages. In the present study, transient serum removal during culture greatly enhanced cardiogenesis, and reduced generat...
Saved in:
| Published in: | Biochemical and biophysical research communications 2007-03, Vol.355 (1), p.47-53 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3 |
| container_end_page | 53 |
| container_issue | 1 |
| container_start_page | 47 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 355 |
| creator | Terami, Hiromi Hidaka, Kyoko Shirai, Manabu Narumiya, Hiromichi Kuroyanagi, Takuo Arai, Yuji Aburatani, Hiroyuki Morisaki, Takayuki |
| description | Cardiogenesis can be induced
in vitro in ES cells, though it is difficult to distinguish cardiac-specific genes, since embryoid bodies simultaneously differentiate into multiple lineages. In the present study, transient serum removal during culture greatly enhanced cardiogenesis, and reduced generation of endothelial and hematopoietic cells. Using DNA microarray analysis of 24 differentiated sample cultures including cardiogenesis-enhanced cells, we successfully selected genes up-regulated in embryoid bodies that had undergone cardiogenic differentiation. Besides contractile protein genes, cardiac transcriptional regulatory genes, such as Nkx2-5, Gata4/5, Mef2c, and Myocd, were primary constituents of the first 100 genes chosen as cardiogenesis-associated genes. Further, whole mount
in situ hybridization analysis of 13 genes containing non-characterized ones confirmed that most of them were specifically expressed in the heart region of mouse embryos from E9.5–10.5. Based on our results, we consider that the present profiling method may be useful to identify novel genes important for cardiac development. |
| doi_str_mv | 10.1016/j.bbrc.2007.01.109 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69036048</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X07001519</els_id><sourcerecordid>69036048</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3</originalsourceid><addsrcrecordid>eNqFkE1r4zAQhkXZ0qYff6CHxae9OZ2RbNmCvSwhbQOFHtpCb0KSR0UhibOSs7T_vnIT2Ft70cfLMy_Dw9gVwhQB5fVyam10Uw7QTAFzpo7YJJ9QcoTqB5sAgCy5wpdTdpbSEgCxkuqEnWLDW6kkn7DF3PvgAm2GwpntsItU9D4_Yxf6V9pQCqk0KfUumIG64jMq6G0bKaX8D5ti_lg4Wq3SBTv2ZpXo8nCfs-eb-dPsrrx_uF3M_tyXTrT1UAri3pqmraw0Hi1ITjVQRUJVgN6RrME4Y0Ai1qIF67FWdWOkEK0VJKw4Z7_2vdvY_91RGvQ6pHEDs6F-l7RUICRU7bcgKokcVZNBvgdd7FOK5PU2hrWJ7xpBj6b1Uo-m9WhaA-ZM5aGfh_adXVP3f-SgNgO_9wBlGf8CRZ1Gz466EMkNuuvDV_0fmC-O0w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19612197</pqid></control><display><type>article</type><title>Efficient capture of cardiogenesis-associated genes expressed in ES cells</title><source>ScienceDirect Journals</source><creator>Terami, Hiromi ; Hidaka, Kyoko ; Shirai, Manabu ; Narumiya, Hiromichi ; Kuroyanagi, Takuo ; Arai, Yuji ; Aburatani, Hiroyuki ; Morisaki, Takayuki</creator><creatorcontrib>Terami, Hiromi ; Hidaka, Kyoko ; Shirai, Manabu ; Narumiya, Hiromichi ; Kuroyanagi, Takuo ; Arai, Yuji ; Aburatani, Hiroyuki ; Morisaki, Takayuki</creatorcontrib><description>Cardiogenesis can be induced
in vitro in ES cells, though it is difficult to distinguish cardiac-specific genes, since embryoid bodies simultaneously differentiate into multiple lineages. In the present study, transient serum removal during culture greatly enhanced cardiogenesis, and reduced generation of endothelial and hematopoietic cells. Using DNA microarray analysis of 24 differentiated sample cultures including cardiogenesis-enhanced cells, we successfully selected genes up-regulated in embryoid bodies that had undergone cardiogenic differentiation. Besides contractile protein genes, cardiac transcriptional regulatory genes, such as Nkx2-5, Gata4/5, Mef2c, and Myocd, were primary constituents of the first 100 genes chosen as cardiogenesis-associated genes. Further, whole mount
in situ hybridization analysis of 13 genes containing non-characterized ones confirmed that most of them were specifically expressed in the heart region of mouse embryos from E9.5–10.5. Based on our results, we consider that the present profiling method may be useful to identify novel genes important for cardiac development.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.01.109</identifier><identifier>PMID: 17286962</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Apoptosis ; Cardiogenesis ; Cell Differentiation ; Cell Line ; Culture Media, Serum-Free ; Development ; Differentiation ; Embryoid body ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - physiology ; Gene expression ; Gene Expression Regulation ; Heart - embryology ; Heart Defects, Congenital - genetics ; Humans ; Mesoderm ; Microarray ; Mutation ; Oligonucleotide Array Sequence Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription Factors - metabolism ; Transcription, Genetic ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 2007-03, Vol.355 (1), p.47-53</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3</citedby><cites>FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17286962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Terami, Hiromi</creatorcontrib><creatorcontrib>Hidaka, Kyoko</creatorcontrib><creatorcontrib>Shirai, Manabu</creatorcontrib><creatorcontrib>Narumiya, Hiromichi</creatorcontrib><creatorcontrib>Kuroyanagi, Takuo</creatorcontrib><creatorcontrib>Arai, Yuji</creatorcontrib><creatorcontrib>Aburatani, Hiroyuki</creatorcontrib><creatorcontrib>Morisaki, Takayuki</creatorcontrib><title>Efficient capture of cardiogenesis-associated genes expressed in ES cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Cardiogenesis can be induced
in vitro in ES cells, though it is difficult to distinguish cardiac-specific genes, since embryoid bodies simultaneously differentiate into multiple lineages. In the present study, transient serum removal during culture greatly enhanced cardiogenesis, and reduced generation of endothelial and hematopoietic cells. Using DNA microarray analysis of 24 differentiated sample cultures including cardiogenesis-enhanced cells, we successfully selected genes up-regulated in embryoid bodies that had undergone cardiogenic differentiation. Besides contractile protein genes, cardiac transcriptional regulatory genes, such as Nkx2-5, Gata4/5, Mef2c, and Myocd, were primary constituents of the first 100 genes chosen as cardiogenesis-associated genes. Further, whole mount
in situ hybridization analysis of 13 genes containing non-characterized ones confirmed that most of them were specifically expressed in the heart region of mouse embryos from E9.5–10.5. Based on our results, we consider that the present profiling method may be useful to identify novel genes important for cardiac development.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Cardiogenesis</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Culture Media, Serum-Free</subject><subject>Development</subject><subject>Differentiation</subject><subject>Embryoid body</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Heart - embryology</subject><subject>Heart Defects, Congenital - genetics</subject><subject>Humans</subject><subject>Mesoderm</subject><subject>Microarray</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r4zAQhkXZ0qYff6CHxae9OZ2RbNmCvSwhbQOFHtpCb0KSR0UhibOSs7T_vnIT2Ft70cfLMy_Dw9gVwhQB5fVyam10Uw7QTAFzpo7YJJ9QcoTqB5sAgCy5wpdTdpbSEgCxkuqEnWLDW6kkn7DF3PvgAm2GwpntsItU9D4_Yxf6V9pQCqk0KfUumIG64jMq6G0bKaX8D5ti_lg4Wq3SBTv2ZpXo8nCfs-eb-dPsrrx_uF3M_tyXTrT1UAri3pqmraw0Hi1ITjVQRUJVgN6RrME4Y0Ai1qIF67FWdWOkEK0VJKw4Z7_2vdvY_91RGvQ6pHEDs6F-l7RUICRU7bcgKokcVZNBvgdd7FOK5PU2hrWJ7xpBj6b1Uo-m9WhaA-ZM5aGfh_adXVP3f-SgNgO_9wBlGf8CRZ1Gz466EMkNuuvDV_0fmC-O0w</recordid><startdate>20070330</startdate><enddate>20070330</enddate><creator>Terami, Hiromi</creator><creator>Hidaka, Kyoko</creator><creator>Shirai, Manabu</creator><creator>Narumiya, Hiromichi</creator><creator>Kuroyanagi, Takuo</creator><creator>Arai, Yuji</creator><creator>Aburatani, Hiroyuki</creator><creator>Morisaki, Takayuki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070330</creationdate><title>Efficient capture of cardiogenesis-associated genes expressed in ES cells</title><author>Terami, Hiromi ; Hidaka, Kyoko ; Shirai, Manabu ; Narumiya, Hiromichi ; Kuroyanagi, Takuo ; Arai, Yuji ; Aburatani, Hiroyuki ; Morisaki, Takayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Cardiogenesis</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Culture Media, Serum-Free</topic><topic>Development</topic><topic>Differentiation</topic><topic>Embryoid body</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Heart - embryology</topic><topic>Heart Defects, Congenital - genetics</topic><topic>Humans</topic><topic>Mesoderm</topic><topic>Microarray</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Terami, Hiromi</creatorcontrib><creatorcontrib>Hidaka, Kyoko</creatorcontrib><creatorcontrib>Shirai, Manabu</creatorcontrib><creatorcontrib>Narumiya, Hiromichi</creatorcontrib><creatorcontrib>Kuroyanagi, Takuo</creatorcontrib><creatorcontrib>Arai, Yuji</creatorcontrib><creatorcontrib>Aburatani, Hiroyuki</creatorcontrib><creatorcontrib>Morisaki, Takayuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Terami, Hiromi</au><au>Hidaka, Kyoko</au><au>Shirai, Manabu</au><au>Narumiya, Hiromichi</au><au>Kuroyanagi, Takuo</au><au>Arai, Yuji</au><au>Aburatani, Hiroyuki</au><au>Morisaki, Takayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient capture of cardiogenesis-associated genes expressed in ES cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-03-30</date><risdate>2007</risdate><volume>355</volume><issue>1</issue><spage>47</spage><epage>53</epage><pages>47-53</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Cardiogenesis can be induced
in vitro in ES cells, though it is difficult to distinguish cardiac-specific genes, since embryoid bodies simultaneously differentiate into multiple lineages. In the present study, transient serum removal during culture greatly enhanced cardiogenesis, and reduced generation of endothelial and hematopoietic cells. Using DNA microarray analysis of 24 differentiated sample cultures including cardiogenesis-enhanced cells, we successfully selected genes up-regulated in embryoid bodies that had undergone cardiogenic differentiation. Besides contractile protein genes, cardiac transcriptional regulatory genes, such as Nkx2-5, Gata4/5, Mef2c, and Myocd, were primary constituents of the first 100 genes chosen as cardiogenesis-associated genes. Further, whole mount
in situ hybridization analysis of 13 genes containing non-characterized ones confirmed that most of them were specifically expressed in the heart region of mouse embryos from E9.5–10.5. Based on our results, we consider that the present profiling method may be useful to identify novel genes important for cardiac development.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17286962</pmid><doi>10.1016/j.bbrc.2007.01.109</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2007-03, Vol.355 (1), p.47-53 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69036048 |
| source | ScienceDirect Journals |
| subjects | Animals Apoptosis Cardiogenesis Cell Differentiation Cell Line Culture Media, Serum-Free Development Differentiation Embryoid body Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Gene expression Gene Expression Regulation Heart - embryology Heart Defects, Congenital - genetics Humans Mesoderm Microarray Mutation Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Transcription Factors - metabolism Transcription, Genetic Transfection |
| title | Efficient capture of cardiogenesis-associated genes expressed in ES cells |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T10%3A02%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efficient%20capture%20of%20cardiogenesis-associated%20genes%20expressed%20in%20ES%20cells&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Terami,%20Hiromi&rft.date=2007-03-30&rft.volume=355&rft.issue=1&rft.spage=47&rft.epage=53&rft.pages=47-53&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2007.01.109&rft_dat=%3Cproquest_cross%3E69036048%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c385t-3e2fba784b6af1b062e50e4e39401fce650acaa06115380bf15957a6338b3e3b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=19612197&rft_id=info:pmid/17286962&rfr_iscdi=true |