Simultaneous determination and pharmacokinetic studies of dihydromyricetin and myricetin in rat plasma by HPLC-DAD after oral administration of Ampelopsis grossedentata decoction

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-12, Vol.860 (1), p.4-9
• Main Authors: Zhang, Yan-song, Zhang, Qing-ying, Li, Li-ying, Wang, Bin, Zhao, Yu-ying, Guo, De-an
• Format: Article
• Language: English
• Subjects: Administration, Oral; Ampelopsis - chemistry; Ampelopsis grossedentata; Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Dihydromyricetin; Drug Stability; Flavonoids - blood; Flavonoids - pharmacokinetics; Flavonols - blood; Flavonols - pharmacokinetics; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC-DAD; Male; Medical sciences; Myricetin; Pharmacokinetics; Pharmacology. Drug treatments; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.07.049

A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous determination of dihydromyricetin ( 1) and myricetin ( 2) in rat plasma after orally administrating the decoction of Ampelopsis grossedentata. Plasma samples were acidified with 0.375% phosphoric acid and extracted with ethyl acetate. Analysis of the extract was performed on reversed-phase C 18 column with a gradient eluent composed of acetonitrile and 0.04% phosphoric acid. The flow rate was kept at 1 ml/min and the detection wavelength was set at 290 and 370 nm for 1 and 2, respectively. The calibration curves were linear in the range of 0.247–4.114 μg/ml and 0.150–2.501 μg/ml for 1 and 2, respectively. The intra-day and inter-day precisions were better than 4.9 and 6.2%, respectively. The limits of detection (LOD) for 1 and 2 in plasma were 21.600 and 52.530 ng/ml, and the limits of quantification (LOQ) were 0.247 and 0.150 μg/ml, respectively. The mean recoveries for 1 and 2 were 92.0 and 93.3%, respectively. The accuracy and precision were well within the acceptable range and R.S.D. of measured rat samples was less than 7.5%. This validated method has been successfully applied in the pharmacokinetics study of dihydromyricetin and myricetin in vivo after orally administrating the decoction of A. grossedentata to rats.