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Fingerprint Reagents with Dual Action: Color and Fluorescence
We define “dual fingerprint reagents” as chemical formulations that produce with latent fingerprints in one stage impressions that are both colored and fluorescent. Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent f...
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Published in: | Journal of forensic sciences 2007-03, Vol.52 (2), p.330-334 |
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creator | Almog, Joseph Levinton-Shamuilov, Genyia Cohen, Yaron Azoury, Myriam |
description | We define “dual fingerprint reagents” as chemical formulations that produce with latent fingerprints in one stage impressions that are both colored and fluorescent. Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent fingerprints on paper is as efficient as the two‐step process beginning with ninhydrin and followed by treatment with metal salt. In the color mode, fingerprint detectability with the two ninhydrin–metal salt reagents (one with zinc chloride and the other with cadmium chloride) is comparable with that of ninhydrin itself, in spite of the difference in color. The sensitivity is significantly higher in the fluorescence mode. To view the latent impressions the exhibits are treated with ninhydrin–metal salt reagents and observed under white light illumination and under fluorescence conditions. Cooling to liquid nitrogen temperature enhances the fluorescence considerably. In the shorter wavelength domain, ninhydrin–metal salt reagents exhibit higher sensitivity than the recently reported dual reagent, genipin. The latter is advantageous, however, in the longer wavelength domain, on paper items with strong self‐fluorescence, such as brown wrapping paper or paper printed with fluorescent ink. Upon reduction of the ninhydrin concentration 10‐fold, ninhydrin–metal salt formulations become purely fluorogenic reagents; no color is noticed but the fluorescence is as intense as with concentrated solutions. Working at lower concentrations is an advantage from ecological and economical viewpoints. |
doi_str_mv | 10.1111/j.1556-4029.2007.00383.x |
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Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent fingerprints on paper is as efficient as the two‐step process beginning with ninhydrin and followed by treatment with metal salt. In the color mode, fingerprint detectability with the two ninhydrin–metal salt reagents (one with zinc chloride and the other with cadmium chloride) is comparable with that of ninhydrin itself, in spite of the difference in color. The sensitivity is significantly higher in the fluorescence mode. To view the latent impressions the exhibits are treated with ninhydrin–metal salt reagents and observed under white light illumination and under fluorescence conditions. Cooling to liquid nitrogen temperature enhances the fluorescence considerably. In the shorter wavelength domain, ninhydrin–metal salt reagents exhibit higher sensitivity than the recently reported dual reagent, genipin. The latter is advantageous, however, in the longer wavelength domain, on paper items with strong self‐fluorescence, such as brown wrapping paper or paper printed with fluorescent ink. Upon reduction of the ninhydrin concentration 10‐fold, ninhydrin–metal salt formulations become purely fluorogenic reagents; no color is noticed but the fluorescence is as intense as with concentrated solutions. Working at lower concentrations is an advantage from ecological and economical viewpoints.</description><identifier>ISSN: 0022-1198</identifier><identifier>EISSN: 1556-4029</identifier><identifier>DOI: 10.1111/j.1556-4029.2007.00383.x</identifier><identifier>PMID: 17316228</identifier><identifier>CODEN: JFSCAS</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Acids ; amino acid reagent ; Cadmium Chloride ; Chemical compounds ; Chlorides ; Color ; colorimetric ; Dermatoglyphics ; fingerprint reagent ; Fingerprinting ; Fluorescence ; fluorogenic ; forensic science ; Forensic sciences ; Humans ; Indicators and Reagents ; Iridoid Glycosides ; Iridoids ; Ninhydrin ; zinc chloride ; Zinc Compounds</subject><ispartof>Journal of forensic sciences, 2007-03, Vol.52 (2), p.330-334</ispartof><rights>Copyright American Society for Testing and Materials Mar 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4623-c07e370a0ad79100aa4f5f629cc9b6d9f0f69ba45d5a90ecd871d04429eacf8b3</citedby><cites>FETCH-LOGICAL-c4623-c07e370a0ad79100aa4f5f629cc9b6d9f0f69ba45d5a90ecd871d04429eacf8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17316228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Almog, Joseph</creatorcontrib><creatorcontrib>Levinton-Shamuilov, Genyia</creatorcontrib><creatorcontrib>Cohen, Yaron</creatorcontrib><creatorcontrib>Azoury, Myriam</creatorcontrib><title>Fingerprint Reagents with Dual Action: Color and Fluorescence</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>We define “dual fingerprint reagents” as chemical formulations that produce with latent fingerprints in one stage impressions that are both colored and fluorescent. Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent fingerprints on paper is as efficient as the two‐step process beginning with ninhydrin and followed by treatment with metal salt. In the color mode, fingerprint detectability with the two ninhydrin–metal salt reagents (one with zinc chloride and the other with cadmium chloride) is comparable with that of ninhydrin itself, in spite of the difference in color. The sensitivity is significantly higher in the fluorescence mode. To view the latent impressions the exhibits are treated with ninhydrin–metal salt reagents and observed under white light illumination and under fluorescence conditions. Cooling to liquid nitrogen temperature enhances the fluorescence considerably. In the shorter wavelength domain, ninhydrin–metal salt reagents exhibit higher sensitivity than the recently reported dual reagent, genipin. The latter is advantageous, however, in the longer wavelength domain, on paper items with strong self‐fluorescence, such as brown wrapping paper or paper printed with fluorescent ink. Upon reduction of the ninhydrin concentration 10‐fold, ninhydrin–metal salt formulations become purely fluorogenic reagents; no color is noticed but the fluorescence is as intense as with concentrated solutions. Working at lower concentrations is an advantage from ecological and economical viewpoints.</description><subject>Acids</subject><subject>amino acid reagent</subject><subject>Cadmium Chloride</subject><subject>Chemical compounds</subject><subject>Chlorides</subject><subject>Color</subject><subject>colorimetric</subject><subject>Dermatoglyphics</subject><subject>fingerprint reagent</subject><subject>Fingerprinting</subject><subject>Fluorescence</subject><subject>fluorogenic</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Iridoid Glycosides</subject><subject>Iridoids</subject><subject>Ninhydrin</subject><subject>zinc chloride</subject><subject>Zinc Compounds</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkVFv0zAUha0JRLvBX0ARD7wlu7YTJ0bwMJW1G5QWoQ14u3IcZ6RLk2InWvvv5y5VkXgBv1xL9ztH9jmEBBQi6s_5KqJJIsIYmIwYQBoB8IxH2xMyPi6ekTEAYyGlMhuRU-dWACCooC_IiKacCsayMfkwrZo7Yze2arrgm1F3pulc8FB1v4KPvaqDC91VbfMumLR1awPVFMG07ltrnDaNNi_J81LVzrw6zDNyO728mVyF8-XsenIxD3UsGA81pIanoEAVqaQASsVlUgomtZa5KGQJpZC5ipMiURKMLrKUFhDHTBqlyyznZ-Tt4Lux7e_euA7XlX9BXavGtL1DIYFLRvk_QR4z8BEkHnzzF7hqe9v4TyCj0vtlIDyUDZC2rXPWlOhzWiu7Qwq4LwJXuM8b93njvgh8KgK3Xvr64N_na1P8ER6S98D7AXioarP7b2P8NF36i5eHg7xyndke5creo0h5muCPxQypkD-_fl98xi_8EcwxpA4</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>Almog, Joseph</creator><creator>Levinton-Shamuilov, Genyia</creator><creator>Cohen, Yaron</creator><creator>Azoury, Myriam</creator><general>Blackwell Publishing Inc</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope><scope>7X8</scope></search><sort><creationdate>200703</creationdate><title>Fingerprint Reagents with Dual Action: Color and Fluorescence</title><author>Almog, Joseph ; Levinton-Shamuilov, Genyia ; Cohen, Yaron ; Azoury, Myriam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4623-c07e370a0ad79100aa4f5f629cc9b6d9f0f69ba45d5a90ecd871d04429eacf8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acids</topic><topic>amino acid reagent</topic><topic>Cadmium Chloride</topic><topic>Chemical compounds</topic><topic>Chlorides</topic><topic>Color</topic><topic>colorimetric</topic><topic>Dermatoglyphics</topic><topic>fingerprint reagent</topic><topic>Fingerprinting</topic><topic>Fluorescence</topic><topic>fluorogenic</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Humans</topic><topic>Indicators and Reagents</topic><topic>Iridoid Glycosides</topic><topic>Iridoids</topic><topic>Ninhydrin</topic><topic>zinc chloride</topic><topic>Zinc Compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Almog, Joseph</creatorcontrib><creatorcontrib>Levinton-Shamuilov, Genyia</creatorcontrib><creatorcontrib>Cohen, Yaron</creatorcontrib><creatorcontrib>Azoury, Myriam</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Almog, Joseph</au><au>Levinton-Shamuilov, Genyia</au><au>Cohen, Yaron</au><au>Azoury, Myriam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fingerprint Reagents with Dual Action: Color and Fluorescence</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2007-03</date><risdate>2007</risdate><volume>52</volume><issue>2</issue><spage>330</spage><epage>334</epage><pages>330-334</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>We define “dual fingerprint reagents” as chemical formulations that produce with latent fingerprints in one stage impressions that are both colored and fluorescent. Solutions containing ninhydrin and group IIb metal salts appear to be true dual reagents. Application of these formulations to latent fingerprints on paper is as efficient as the two‐step process beginning with ninhydrin and followed by treatment with metal salt. In the color mode, fingerprint detectability with the two ninhydrin–metal salt reagents (one with zinc chloride and the other with cadmium chloride) is comparable with that of ninhydrin itself, in spite of the difference in color. The sensitivity is significantly higher in the fluorescence mode. To view the latent impressions the exhibits are treated with ninhydrin–metal salt reagents and observed under white light illumination and under fluorescence conditions. Cooling to liquid nitrogen temperature enhances the fluorescence considerably. In the shorter wavelength domain, ninhydrin–metal salt reagents exhibit higher sensitivity than the recently reported dual reagent, genipin. The latter is advantageous, however, in the longer wavelength domain, on paper items with strong self‐fluorescence, such as brown wrapping paper or paper printed with fluorescent ink. Upon reduction of the ninhydrin concentration 10‐fold, ninhydrin–metal salt formulations become purely fluorogenic reagents; no color is noticed but the fluorescence is as intense as with concentrated solutions. Working at lower concentrations is an advantage from ecological and economical viewpoints.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>17316228</pmid><doi>10.1111/j.1556-4029.2007.00383.x</doi><tpages>5</tpages></addata></record> |
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subjects | Acids amino acid reagent Cadmium Chloride Chemical compounds Chlorides Color colorimetric Dermatoglyphics fingerprint reagent Fingerprinting Fluorescence fluorogenic forensic science Forensic sciences Humans Indicators and Reagents Iridoid Glycosides Iridoids Ninhydrin zinc chloride Zinc Compounds |
title | Fingerprint Reagents with Dual Action: Color and Fluorescence |
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