Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an opt...

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Bibliographic Details
Published in:Optics letters 2007-12, Vol.32 (23), p.3408-3410
Main Authors: OWEN, Dylan M, AUKSORIUS, Egidijus, MANNING, Hugh B, TALBOT, Clifford B, DE BEULE, Pieter A. A, DUNSBY, Christopher, NEIL, Mark A. A, FRENCH, Paul M. W
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Collagen - chemistry
Collagen - ultrastructure
Convallaria - cytology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Fundamental areas of phenomenology (including applications)
General aspects, investigation technics, apparatus
Image forming and processing
Image Processing, Computer-Assisted - instrumentation
Image Processing, Computer-Assisted - methods
Imaging and optical processing
Imaging detectors and sensors
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Microscopy, Ultraviolet - instrumentation
Microscopy, Ultraviolet - methods
Optical elements, devices, and systems
Optics
Physics
Rats
Tail - cytology
Tissues, organs and organisms biophysics
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Description
Summary:We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.32.003408