Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

• Published in: Journal of bioscience and bioengineering 2005-11, Vol.100 (5), p.511-516
• Main Authors: Takata, Maki K., Yamaguchi, Fuminori, Nakanose, Koichi, Watanabe, Yasuo, Hatano, Naoya, Tsukamoto, Ikuko, Nagata, Mitsuhiro, Izumori, Ken, Tokuda, Masaaki
• Format: Article
• Language: English
• Subjects: 6-hydroxydopamine (6-OHDA); Adrenergic Agents - pharmacology; Animals; antioxidant; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; APOPTOSE; APOPTOSIS; Apoptosis - drug effects; Biological and medical sciences; Biotechnology; D-psicose; DOPAMINA; DOPAMINE; Fructose - pharmacology; Fundamental and applied biological sciences. Psychology; Glutathione - biosynthesis; Humans; NEUROFISIOLOGIA; NEUROPHYSIOLOGIE; NEUROPHYSIOLOGY; Neuroprotective Agents - pharmacology; Oxidopamine - pharmacology; Parkinson Disease - drug therapy; Parkinson Disease - metabolism; Parkinson’s disease (PD); PC12 Cells; rare sugars; Rats; Up-Regulation - drug effects
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.100.511

We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson’s disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.